2D and 3D in vitro assays to quantify the invasive behavior of glioblastoma stem cells in response to SDF-1α

Invasion is a hallmark of cancer and therefore in vitro invasion assays are important tools in cancer research. We aimed to describe in vitro 2D transwell assays and 3D spheroid assays to quantitatively determine the invasive behavior of glioblastoma stem cells in response to the chemoattractant SDF...

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Bibliographic Details
Main Authors: Vashendriya VV Hira, Barbara Breznik, Cornelis JF Van Noorden, Tamara Lah, Remco J Molenaar
Format: Article
Language:English
Published: Taylor & Francis Group 2020-11-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2020-0046