An Efficient Protocol for Model Legume Root Protoplast Isolation and Transformation

Transient gene expression systems using protoplasts have been widely used for rapid functional characterization of genes and high-throughput analysis in many model and crop species. Here, we describe a simplified and highly efficient root protoplast isolation and transient expression system in the m...

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Bibliographic Details
Main Authors: Ning Jia, Yali Zhu, Fang Xie
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2018.00670/full