Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured Ham
<i>Listeria monocytogenes</i> population and the expression patterns of three virulence (<i>plcA</i>, <i>hly</i>, and <i>iap</i>) and one stress-related (<i>sigB</i>) genes in dry-cured ham with different water activity (a<sub>w</s...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/9/8/1092 |
| _version_ | 1797559123740459008 |
|---|---|
| author | Aida Pérez-Baltar Alberto Alía Alicia Rodríguez Juan José Córdoba Margarita Medina Raquel Montiel |
| author_facet | Aida Pérez-Baltar Alberto Alía Alicia Rodríguez Juan José Córdoba Margarita Medina Raquel Montiel |
| author_sort | Aida Pérez-Baltar |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> population and the expression patterns of three virulence (<i>plcA</i>, <i>hly</i>, and <i>iap</i>) and one stress-related (<i>sigB</i>) genes in dry-cured ham with different water activity (a<sub>w</sub>) values (0.92, 0.88, and 0.84) and treated with high pressure processing (HPP, 450 MPa/10 min and 600 MPa/5 min) were monitored throughout 30 days (d) at 4 °C. The antimicrobial effect of HPP at 600 MPa against <i>L. monocytogenes</i> S4-2 (serotype 1/2b) and S12-1 (serotype 1/2c) was greater in dry-cured ham with a<sub>w</sub> values of 0.92, with reductions of 2.5 and 2.8 log units, respectively. The efficacy of HPP treatments decreased at lower a<sub>w</sub> values. Regarding gene expression, <i>L. monocytogenes</i> strains responded differently to HPP. For strain S4-2, the four target genes were generally overexpressed in dry-cured ham immediately after HPP treatments at the three a<sub>w</sub> values investigated, although the extent of this induction was lower in the samples pressurized at 600 MPa and with a<sub>w</sub> values of 0.84. For strain S12-1, the expression of all target genes was repressed at the three a<sub>w</sub> values investigated. The antimicrobial efficacy of HPP against <i>L. monocytogenes</i> could be compromised by low a<sub>w</sub> values in food products. However, no growth of HPP-survival cells was observed during refrigerated storage in low-a<sub>w</sub> dry-cured ham, and the overexpression of virulence and stress-related genes decreased. |
| first_indexed | 2024-03-10T17:40:00Z |
| format | Article |
| id | doaj.art-941b610e895d43a680c972019cd56438 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T17:40:00Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-941b610e895d43a680c972019cd564382023-11-20T09:43:22ZengMDPI AGFoods2304-81582020-08-0198109210.3390/foods9081092Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured HamAida Pérez-Baltar0Alberto Alía1Alicia Rodríguez2Juan José Córdoba3Margarita Medina4Raquel Montiel5Departamento de Tecnología de Alimentos, INIA, Carretera de La Coruña Km 7, 28040 Madrid, SpainInstituto Universitario de Investigación de Carne y Productos Cárnicos, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad, s/n., 10003 Cáceres, SpainInstituto Universitario de Investigación de Carne y Productos Cárnicos, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad, s/n., 10003 Cáceres, SpainInstituto Universitario de Investigación de Carne y Productos Cárnicos, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad, s/n., 10003 Cáceres, SpainDepartamento de Tecnología de Alimentos, INIA, Carretera de La Coruña Km 7, 28040 Madrid, SpainDepartamento de Tecnología de Alimentos, INIA, Carretera de La Coruña Km 7, 28040 Madrid, Spain<i>Listeria monocytogenes</i> population and the expression patterns of three virulence (<i>plcA</i>, <i>hly</i>, and <i>iap</i>) and one stress-related (<i>sigB</i>) genes in dry-cured ham with different water activity (a<sub>w</sub>) values (0.92, 0.88, and 0.84) and treated with high pressure processing (HPP, 450 MPa/10 min and 600 MPa/5 min) were monitored throughout 30 days (d) at 4 °C. The antimicrobial effect of HPP at 600 MPa against <i>L. monocytogenes</i> S4-2 (serotype 1/2b) and S12-1 (serotype 1/2c) was greater in dry-cured ham with a<sub>w</sub> values of 0.92, with reductions of 2.5 and 2.8 log units, respectively. The efficacy of HPP treatments decreased at lower a<sub>w</sub> values. Regarding gene expression, <i>L. monocytogenes</i> strains responded differently to HPP. For strain S4-2, the four target genes were generally overexpressed in dry-cured ham immediately after HPP treatments at the three a<sub>w</sub> values investigated, although the extent of this induction was lower in the samples pressurized at 600 MPa and with a<sub>w</sub> values of 0.84. For strain S12-1, the expression of all target genes was repressed at the three a<sub>w</sub> values investigated. The antimicrobial efficacy of HPP against <i>L. monocytogenes</i> could be compromised by low a<sub>w</sub> values in food products. However, no growth of HPP-survival cells was observed during refrigerated storage in low-a<sub>w</sub> dry-cured ham, and the overexpression of virulence and stress-related genes decreased.https://www.mdpi.com/2304-8158/9/8/1092<i>L. monocytogenes</i>high pressure processingcured meatqPCRvirulence and stress-related genes |
| spellingShingle | Aida Pérez-Baltar Alberto Alía Alicia Rodríguez Juan José Córdoba Margarita Medina Raquel Montiel Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured Ham Foods <i>L. monocytogenes</i> high pressure processing cured meat qPCR virulence and stress-related genes |
| title | Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured Ham |
| title_full | Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured Ham |
| title_fullStr | Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured Ham |
| title_full_unstemmed | Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured Ham |
| title_short | Impact of Water Activity on the Inactivation and Gene Expression of <i>Listeria</i> <i>monocytogenes</i> during Refrigerated Storage of Pressurized Dry-Cured Ham |
| title_sort | impact of water activity on the inactivation and gene expression of i listeria i i monocytogenes i during refrigerated storage of pressurized dry cured ham |
| topic | <i>L. monocytogenes</i> high pressure processing cured meat qPCR virulence and stress-related genes |
| url | https://www.mdpi.com/2304-8158/9/8/1092 |
| work_keys_str_mv | AT aidaperezbaltar impactofwateractivityontheinactivationandgeneexpressionofilisteriaiimonocytogenesiduringrefrigeratedstorageofpressurizeddrycuredham AT albertoalia impactofwateractivityontheinactivationandgeneexpressionofilisteriaiimonocytogenesiduringrefrigeratedstorageofpressurizeddrycuredham AT aliciarodriguez impactofwateractivityontheinactivationandgeneexpressionofilisteriaiimonocytogenesiduringrefrigeratedstorageofpressurizeddrycuredham AT juanjosecordoba impactofwateractivityontheinactivationandgeneexpressionofilisteriaiimonocytogenesiduringrefrigeratedstorageofpressurizeddrycuredham AT margaritamedina impactofwateractivityontheinactivationandgeneexpressionofilisteriaiimonocytogenesiduringrefrigeratedstorageofpressurizeddrycuredham AT raquelmontiel impactofwateractivityontheinactivationandgeneexpressionofilisteriaiimonocytogenesiduringrefrigeratedstorageofpressurizeddrycuredham |