Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds
Abstract The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-posit...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-02388-8 |
| _version_ | 1818401884357001216 |
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| author | T. D. Jayasena Kaluarachchi Paul M. Campbell Rajitha Wickremasinghe Shalindra Ranasinghe Renu Wickremasinghe Surangi Yasawardene Hiromel De Silva Chandrani Menike M. C. K. Jayarathne Subodha Jayathilake Ayomi Dilhari Andrew J. McBain Manjula M. Weerasekera |
| author_facet | T. D. Jayasena Kaluarachchi Paul M. Campbell Rajitha Wickremasinghe Shalindra Ranasinghe Renu Wickremasinghe Surangi Yasawardene Hiromel De Silva Chandrani Menike M. C. K. Jayarathne Subodha Jayathilake Ayomi Dilhari Andrew J. McBain Manjula M. Weerasekera |
| author_sort | T. D. Jayasena Kaluarachchi |
| collection | DOAJ |
| description | Abstract The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-positive according to fluorescence in situ hybridization. Biopsies of biofilm-positive lesions were dominated by Pseudomonas, class Bacilli and Enterobacteriaceae and distinguished by significantly lower community evenness. Higher relative abundance of a class Bacilli OTU was detected in wound swabs versus contralateral skin. Wound swabs and biopsies had significantly distinct microbiome profiles and lower diversity compared to unaffected skin. Greater abundances of potentially pathogenic organisms were observed in wet ulcers, lesions with high parasite loads and large wounds. In summary, more than half of L. donovani associated CL wounds harboured biofilms and the wounds exhibited a distinct, less diverse, microbiome than unaffected skin. |
| first_indexed | 2024-12-14T07:59:33Z |
| format | Article |
| id | doaj.art-943638463967409aa9b8f30715a6ea94 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T07:59:33Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-943638463967409aa9b8f30715a6ea942022-12-21T23:10:28ZengNature PortfolioScientific Reports2045-23222021-11-0111111410.1038/s41598-021-02388-8Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis woundsT. D. Jayasena Kaluarachchi0Paul M. Campbell1Rajitha Wickremasinghe2Shalindra Ranasinghe3Renu Wickremasinghe4Surangi Yasawardene5Hiromel De Silva6Chandrani Menike7M. C. K. Jayarathne8Subodha Jayathilake9Ayomi Dilhari10Andrew J. McBain11Manjula M. Weerasekera12Department of Parasitology, Faculty of Medical Sciences, University of Sri JayewardenepuraDivision of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of ManchesterDepartment of Public Health, Faculty of Medicine, University of KelaniyaDepartment of Parasitology, Faculty of Medical Sciences, University of Sri JayewardenepuraDepartment of Parasitology, Faculty of Medical Sciences, University of Sri JayewardenepuraDepartment of Anatomy, Faculty of Medical Sciences, University of Sri JayewardenepuraDermatology Unit, Base HospitalDepartment of Parasitology, Faculty of Medical Sciences, University of Sri JayewardenepuraDepartment of Family Medicine, Faculty of Medical Sciences, University of Sri JayewardenepuraDepartment of Pathology, Faculty of Medical Sciences, University of Sri JayewardenepuraDepartment of Basic Sciences, Faculty of Allied Health Sciences, University of Sri JayewardenepuraDivision of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of ManchesterDepartment of Microbiology, Faculty of Medical Sciences, University of Sri JayewardenepuraAbstract The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-positive according to fluorescence in situ hybridization. Biopsies of biofilm-positive lesions were dominated by Pseudomonas, class Bacilli and Enterobacteriaceae and distinguished by significantly lower community evenness. Higher relative abundance of a class Bacilli OTU was detected in wound swabs versus contralateral skin. Wound swabs and biopsies had significantly distinct microbiome profiles and lower diversity compared to unaffected skin. Greater abundances of potentially pathogenic organisms were observed in wet ulcers, lesions with high parasite loads and large wounds. In summary, more than half of L. donovani associated CL wounds harboured biofilms and the wounds exhibited a distinct, less diverse, microbiome than unaffected skin.https://doi.org/10.1038/s41598-021-02388-8 |
| spellingShingle | T. D. Jayasena Kaluarachchi Paul M. Campbell Rajitha Wickremasinghe Shalindra Ranasinghe Renu Wickremasinghe Surangi Yasawardene Hiromel De Silva Chandrani Menike M. C. K. Jayarathne Subodha Jayathilake Ayomi Dilhari Andrew J. McBain Manjula M. Weerasekera Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds Scientific Reports |
| title | Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds |
| title_full | Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds |
| title_fullStr | Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds |
| title_full_unstemmed | Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds |
| title_short | Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds |
| title_sort | distinct microbiome profiles and biofilms in leishmania donovani driven cutaneous leishmaniasis wounds |
| url | https://doi.org/10.1038/s41598-021-02388-8 |
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