Seeing beyond the blot: A critical look at assumptions and raw data interpretation in Western blotting

Seeing beyond the blot: A critical look at assumptions and raw data interpretation in Western blotting

Rapid advancements in technology refine our understanding of intricate biological processes, but a crucial emphasis remains on understanding the assumptions and sources of uncertainty underlying biological measurements. This is particularly critical in cell signaling research, where a quantitative u...

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Bibliographic Details
Main Authors: DeNies Maxwell S., Liu Allen P., Schnell Santiago
Format: Article
Language:English
Published: De Gruyter 2024-04-01
Series:Biomolecular Concepts
Subjects:
rigor
reproducibility
undergraduate and graduate research education
experimental design
biometrology
quantitative biology
western blots
protein abundance
post-translational modification
cell signaling
Online Access:https://doi.org/10.1515/bmc-2022-0047

Internet

https://doi.org/10.1515/bmc-2022-0047

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