Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway
Unlike mammals, fish express two type II interferons, IFNγ and fish‐specific IFNγ (IFNγ‐related or IFNγrel). We previously reported the presence of two IFNγrel genes, IFNγrel 1 and IFNγrel 2, which exhibit potent antiviral activity in the Ginbuna crucian carp, Carassius auratus langsdorfii. We also...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-04-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13769 |
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| author | Yasuhiro Shibasaki Takeshi Yabu Hajime Shiba Tadaaki Moritomo Nobuhiro Mano Teruyuki Nakanishi |
| author_facet | Yasuhiro Shibasaki Takeshi Yabu Hajime Shiba Tadaaki Moritomo Nobuhiro Mano Teruyuki Nakanishi |
| author_sort | Yasuhiro Shibasaki |
| collection | DOAJ |
| description | Unlike mammals, fish express two type II interferons, IFNγ and fish‐specific IFNγ (IFNγ‐related or IFNγrel). We previously reported the presence of two IFNγrel genes, IFNγrel 1 and IFNγrel 2, which exhibit potent antiviral activity in the Ginbuna crucian carp, Carassius auratus langsdorfii. We also found that IFNγrel 1 increased allograft rejection; however, the IFNγrel 1 receptor(s) and signaling pathways underlying this process have not yet been elucidated. In this study, we examined the unique signaling mechanism of IFNγrel 1 and its receptors. The phosphorylation and transcriptional activation of STAT6 in response to recombinant Ginbuna IFNγrel 1 (rgIFNγrel 1) was observed in Ginbuna‐derived cells. Binding of rgIFNγrel 1 to Class II cytokine receptor family members (Crfbs), Crfb5 and Crfb17, which are also known as IFNAR1 and IFNGR1‐1, respectively, was detected by flow cytometry. Expression of the IFNγrel 1‐inducible antiviral gene, Isg15, was highest in Crfb5‐ and Crfb17‐overexpressing GTS9 cells. Dimerization of Crfb5 and Crfb17 was detected by chemical crosslinking. The results indicate that IFNγrel 1 activates Stat6 through an interaction with unique pairs of receptors, Crfb5 and Crfb17. Indeed, this cascade is distinct from not only that of IFNγ but also that of known IFNs in other vertebrates. IFNs may be classified by their receptor and signal transduction pathways. Taken together, IFNγrel 1 may be classified as a novel type of IFN family member in vertebrates. Our findings provide important information on interferon gene evolution in bony fish. |
| first_indexed | 2024-04-24T14:06:46Z |
| format | Article |
| id | doaj.art-944be67fc39e4b24b1b2bd1a4d02aa6e |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-24T14:06:46Z |
| publishDate | 2024-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-944be67fc39e4b24b1b2bd1a4d02aa6e2024-04-03T10:12:36ZengWileyFEBS Open Bio2211-54632024-04-0114453254410.1002/2211-5463.13769Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathwayYasuhiro Shibasaki0Takeshi Yabu1Hajime Shiba2Tadaaki Moritomo3Nobuhiro Mano4Teruyuki Nakanishi5College of Bioresource Sciences Nihon University Fujisawa JapanCollege of Bioresource Sciences Nihon University Fujisawa JapanCollege of Bioresource Sciences Nihon University Fujisawa JapanCollege of Bioresource Sciences Nihon University Fujisawa JapanCollege of Bioresource Sciences Nihon University Fujisawa JapanCollege of Bioresource Sciences Nihon University Fujisawa JapanUnlike mammals, fish express two type II interferons, IFNγ and fish‐specific IFNγ (IFNγ‐related or IFNγrel). We previously reported the presence of two IFNγrel genes, IFNγrel 1 and IFNγrel 2, which exhibit potent antiviral activity in the Ginbuna crucian carp, Carassius auratus langsdorfii. We also found that IFNγrel 1 increased allograft rejection; however, the IFNγrel 1 receptor(s) and signaling pathways underlying this process have not yet been elucidated. In this study, we examined the unique signaling mechanism of IFNγrel 1 and its receptors. The phosphorylation and transcriptional activation of STAT6 in response to recombinant Ginbuna IFNγrel 1 (rgIFNγrel 1) was observed in Ginbuna‐derived cells. Binding of rgIFNγrel 1 to Class II cytokine receptor family members (Crfbs), Crfb5 and Crfb17, which are also known as IFNAR1 and IFNGR1‐1, respectively, was detected by flow cytometry. Expression of the IFNγrel 1‐inducible antiviral gene, Isg15, was highest in Crfb5‐ and Crfb17‐overexpressing GTS9 cells. Dimerization of Crfb5 and Crfb17 was detected by chemical crosslinking. The results indicate that IFNγrel 1 activates Stat6 through an interaction with unique pairs of receptors, Crfb5 and Crfb17. Indeed, this cascade is distinct from not only that of IFNγ but also that of known IFNs in other vertebrates. IFNs may be classified by their receptor and signal transduction pathways. Taken together, IFNγrel 1 may be classified as a novel type of IFN family member in vertebrates. Our findings provide important information on interferon gene evolution in bony fish.https://doi.org/10.1002/2211-5463.13769cytokine receptorIFNγ‐relatedsignal transductionSTATtype II interferon |
| spellingShingle | Yasuhiro Shibasaki Takeshi Yabu Hajime Shiba Tadaaki Moritomo Nobuhiro Mano Teruyuki Nakanishi Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway FEBS Open Bio cytokine receptor IFNγ‐related signal transduction STAT type II interferon |
| title | Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway |
| title_full | Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway |
| title_fullStr | Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway |
| title_full_unstemmed | Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway |
| title_short | Characterization of fish‐specific IFNγ‐related binding with a unique receptor complex and signaling through a novel pathway |
| title_sort | characterization of fish specific ifnγ related binding with a unique receptor complex and signaling through a novel pathway |
| topic | cytokine receptor IFNγ‐related signal transduction STAT type II interferon |
| url | https://doi.org/10.1002/2211-5463.13769 |
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