A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences.

A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences.

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To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive T-cell clones it is necessary to develop convenient and rapid cloning strategies for the generation of TCR expression constructs. Herein, we describe a pDONR™221 vector backbone allowing to generate Gateway™ com...

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Bibliographic Details
Main Authors: Korbinian N Kropp, Tim J Schäufele, Martina Fatho, Michael Volkmar, Roland Conradi, Matthias Theobald, Thomas Wölfel, Catherine Wölfel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0238875

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https://doi.org/10.1371/journal.pone.0238875

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