Exploring the Potential of CRISPR-Cas9 Under Challenging Conditions: Facing High-Copy Plasmids and Counteracting Beta-Lactam Resistance in Clinical Strains of Enterobacteriaceae

Exploring the Potential of CRISPR-Cas9 Under Challenging Conditions: Facing High-Copy Plasmids and Counteracting Beta-Lactam Resistance in Clinical Strains of Enterobacteriaceae

The antimicrobial resistance (AMR) crisis urgently requires countermeasures for reducing the dissemination of plasmid-borne resistance genes. Of particular concern are opportunistic pathogens of Enterobacteriaceae. One innovative approach is the CRISPR-Cas9 system which has recently been used for pl...

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Bibliographic Details
Main Authors: Thaysa Leite Tagliaferri, Natália Rocha Guimarães, Marcella de Paula Martins Pereira, Liza Figueiredo Felicori Vilela, Hans-Peter Horz, Simone Gonçalves dos Santos, Tiago Antônio de Oliveira Mendes
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-04-01
Series:Frontiers in Microbiology
Subjects:
CRISPR-Cas9
antimicrobial resistance
blaTEM
re-sensitization
resistance reduction
plasmid maintenance
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.00578/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2020.00578/full

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