Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen
Summary: This study investigates the efficacy of proteomic analysis of human remains to identify active infections in the past through the detection of pathogens and the host response to infection. We advance leprosy as a case study due to the sequestering of sufferers in leprosaria and the suggesti...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422400885X |
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| author | Shevan Wilkin Liam T. Lanigan Nuria Montes Mukul Sharma Charlotte Avanzi Donikë Sejdiu Kerttu Majander Saskia Pfrengle Yun Chiang Laura Kunz Antje Dittmann Frank Rühli Pushpendra Singh Maria Fontanals Coll Matthew J. Collins Alberto J. Taurozzi Verena J. Schuenemann |
| author_facet | Shevan Wilkin Liam T. Lanigan Nuria Montes Mukul Sharma Charlotte Avanzi Donikë Sejdiu Kerttu Majander Saskia Pfrengle Yun Chiang Laura Kunz Antje Dittmann Frank Rühli Pushpendra Singh Maria Fontanals Coll Matthew J. Collins Alberto J. Taurozzi Verena J. Schuenemann |
| author_sort | Shevan Wilkin |
| collection | DOAJ |
| description | Summary: This study investigates the efficacy of proteomic analysis of human remains to identify active infections in the past through the detection of pathogens and the host response to infection. We advance leprosy as a case study due to the sequestering of sufferers in leprosaria and the suggestive skeletal lesions that can result from the disease. Here we present a sequential enzyme extraction protocol, using trypsin followed by ProAlanase, to reduce the abundance of collagen peptides and in so doing increase the detection of non-collagenous proteins. Through our study of five individuals from an 11th to 18th century leprosarium, as well as four from a contemporaneous non-leprosy associated cemetery in Barcelona, we show that samples from 2 out of 5 leprosarium individuals extracted with the sequential digestion methodology contain numerous host immune proteins associated with modern leprosy. In contrast, individuals from the non-leprosy associated cemetery and all samples extracted with a trypsin-only protocol did not. Through this study, we advance a palaeoproteomic methodology to gain insights into the health of archaeological individuals and take a step toward a proteomics-based method to study immune responses in past populations. |
| first_indexed | 2024-04-24T07:37:31Z |
| format | Article |
| id | doaj.art-9477f89466d14e009bbde0fb540ae0d2 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-24T07:37:31Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9477f89466d14e009bbde0fb540ae0d22024-04-20T04:17:44ZengElsevieriScience2589-00422024-05-01275109663Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagenShevan Wilkin0Liam T. Lanigan1Nuria Montes2Mukul Sharma3Charlotte Avanzi4Donikë Sejdiu5Kerttu Majander6Saskia Pfrengle7Yun Chiang8Laura Kunz9Antje Dittmann10Frank Rühli11Pushpendra Singh12Maria Fontanals Coll13Matthew J. Collins14Alberto J. Taurozzi15Verena J. Schuenemann16Institute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland; Max Planck Institute of Geoanthropology, Jena, Germany; Australian Research Centre for Human Evolution, Griffith University, Brisbane, QLD, Australia; Corresponding authorGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Corresponding authorUnitat d'Antropologia Biològica, Departament de Biologia Animal, Biologia Vegetal i Ecologia, Universitat Autònoma de Barcelona, Barcelona, SpainMicrobial Pathogenesis and Genomics, National Institute of Research in Tribal Health, Jabalpur, MP, IndiaDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USAInstitute of Evolutionary Medicine, University of Zurich, Zurich, SwitzerlandInstitute of Evolutionary Medicine, University of Zurich, Zurich, SwitzerlandInstitute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland; Institute for Archaeological Sciences, University of Tübingen, Tübingen, GermanyGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkFunctional Genomics Center Zurich, University of Zurich, Zurich, SwitzerlandFunctional Genomics Center Zurich, University of Zurich, Zurich, SwitzerlandInstitute of Evolutionary Medicine, University of Zurich, Zurich, SwitzerlandMicrobial Pathogenesis and Genomics, National Institute of Research in Tribal Health, Jabalpur, MP, India; Model Rural Health Research Unit, Badoni, Datia (MP), IndiaBioArCh - Department of Archaeology, University of York, York, UKGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; McDonald Institute for Archaeological Research, University of Cambridge, West Tower, Downing St, Cambridge CB2 3ER, UKGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Corresponding authorInstitute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland; Corresponding authorSummary: This study investigates the efficacy of proteomic analysis of human remains to identify active infections in the past through the detection of pathogens and the host response to infection. We advance leprosy as a case study due to the sequestering of sufferers in leprosaria and the suggestive skeletal lesions that can result from the disease. Here we present a sequential enzyme extraction protocol, using trypsin followed by ProAlanase, to reduce the abundance of collagen peptides and in so doing increase the detection of non-collagenous proteins. Through our study of five individuals from an 11th to 18th century leprosarium, as well as four from a contemporaneous non-leprosy associated cemetery in Barcelona, we show that samples from 2 out of 5 leprosarium individuals extracted with the sequential digestion methodology contain numerous host immune proteins associated with modern leprosy. In contrast, individuals from the non-leprosy associated cemetery and all samples extracted with a trypsin-only protocol did not. Through this study, we advance a palaeoproteomic methodology to gain insights into the health of archaeological individuals and take a step toward a proteomics-based method to study immune responses in past populations.http://www.sciencedirect.com/science/article/pii/S258900422400885XBiochemistryBiochemistry applicationsBiochemistry methodsMedical biochemistryProteomics |
| spellingShingle | Shevan Wilkin Liam T. Lanigan Nuria Montes Mukul Sharma Charlotte Avanzi Donikë Sejdiu Kerttu Majander Saskia Pfrengle Yun Chiang Laura Kunz Antje Dittmann Frank Rühli Pushpendra Singh Maria Fontanals Coll Matthew J. Collins Alberto J. Taurozzi Verena J. Schuenemann Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen iScience Biochemistry Biochemistry applications Biochemistry methods Medical biochemistry Proteomics |
| title | Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen |
| title_full | Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen |
| title_fullStr | Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen |
| title_full_unstemmed | Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen |
| title_short | Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen |
| title_sort | sequential trypsin and proalanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen |
| topic | Biochemistry Biochemistry applications Biochemistry methods Medical biochemistry Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S258900422400885X |
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