Multiplexed CRISPR-Cas9 protocol for large transgene integration into the Schistosoma mansoni genome

Multiplexed CRISPR-Cas9 protocol for large transgene integration into the Schistosoma mansoni genome

Summary: Precise, on-target CRISPR-Cas9 genome editing has been shown in Schistosoma mansoni, involving both non-homology end joining and homology-directed repair pathways. Here, we present a multiplexed CRISPR-Cas9 protocol for large transgene integration into the S. mansoni genome. We describe ste...

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Bibliographic Details
Main Authors: Wannaporn Ittiprasert, Max M. Moescheid, Victoria H. Mann, Paul J. Brindley
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Model Organisms
Molecular Biology
Gene Expression
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000510

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http://www.sciencedirect.com/science/article/pii/S2666166724000510

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