Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation
<p>Abstract</p> <p>Background</p> <p>Clara cell protein (CC16), the main secretory product of bronchiolar Clara cells, plays an important protective role in the respiratory tract against oxidative stress and inflammation. The purpose of the study was to investigate the...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2010-04-01
|
Series: | Particle and Fibre Toxicology |
Online Access: | http://www.particleandfibretoxicology.com/content/7/1/11 |
_version_ | 1818065363064061952 |
---|---|
author | Schulz Holger Mempel Martin Blume Cornelia Karg Erwin Takenaka Shinji van Miert Erik Weichenmeier Ingrid Alessandrini Francesca Bernard Alfred Behrendt Heidrun |
author_facet | Schulz Holger Mempel Martin Blume Cornelia Karg Erwin Takenaka Shinji van Miert Erik Weichenmeier Ingrid Alessandrini Francesca Bernard Alfred Behrendt Heidrun |
author_sort | Schulz Holger |
collection | DOAJ |
description | <p>Abstract</p> <p>Background</p> <p>Clara cell protein (CC16), the main secretory product of bronchiolar Clara cells, plays an important protective role in the respiratory tract against oxidative stress and inflammation. The purpose of the study was to investigate the role of elemental carbon ultrafine particles (EC-UFP)-induced oxidative stress on Clara cells and CC16 in a mouse model of allergic lung inflammation.</p> <p>Methods</p> <p>Ovalbumin (OVA)-sensitized mice were exposed to EC-UFP (507 μg/m<sup>3 </sup>for 24 h) or filtered air immediately prior to allergen challenge and systemically treated with N-acetylcysteine (NAC) or vehicle prior and during EC-UFP inhalation. CC16 was measured up to one week after allergen challenge in bronchoalveolar lavage fluid (BALF) and in serum. The relative expression of CC16 and TNF-α mRNA were measured in lung homogenates. A morphometrical analysis of mucus hypersecretion and electron microscopy served to investigate goblet cell metaplasia and Clara cell morphological alterations.</p> <p>Results</p> <p>In non sensitized mice EC-UFP inhalation caused alterations in CC16 concentration, both at protein and mRNA level, and induced Clara cell hyperplasia. In sensitized mice, inhalation of EC-UFP prior to OVA challenge caused most significant alterations of BALF and serum CC16 concentration, BALF total protein and TNF-α relative expression compared to relevant controls; their Clara cells displayed the strongest morphological alterations and strongest goblet cell metaplasia occurred in the small airways. NAC strongly reduced both functional and morphological alterations of Clara cells.</p> <p>Conclusion</p> <p>Our findings demonstrate that oxidative stress plays an important role in EC-UFP-induced augmentation of functional and morphological alterations of Clara cells in allergic lung inflammation.</p> |
first_indexed | 2024-12-10T14:50:42Z |
format | Article |
id | doaj.art-948c1677062647bcb968f2ef0415ab8d |
institution | Directory Open Access Journal |
issn | 1743-8977 |
language | English |
last_indexed | 2024-12-10T14:50:42Z |
publishDate | 2010-04-01 |
publisher | BMC |
record_format | Article |
series | Particle and Fibre Toxicology |
spelling | doaj.art-948c1677062647bcb968f2ef0415ab8d2022-12-22T01:44:27ZengBMCParticle and Fibre Toxicology1743-89772010-04-01711110.1186/1743-8977-7-11Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammationSchulz HolgerMempel MartinBlume CorneliaKarg ErwinTakenaka Shinjivan Miert ErikWeichenmeier IngridAlessandrini FrancescaBernard AlfredBehrendt Heidrun<p>Abstract</p> <p>Background</p> <p>Clara cell protein (CC16), the main secretory product of bronchiolar Clara cells, plays an important protective role in the respiratory tract against oxidative stress and inflammation. The purpose of the study was to investigate the role of elemental carbon ultrafine particles (EC-UFP)-induced oxidative stress on Clara cells and CC16 in a mouse model of allergic lung inflammation.</p> <p>Methods</p> <p>Ovalbumin (OVA)-sensitized mice were exposed to EC-UFP (507 μg/m<sup>3 </sup>for 24 h) or filtered air immediately prior to allergen challenge and systemically treated with N-acetylcysteine (NAC) or vehicle prior and during EC-UFP inhalation. CC16 was measured up to one week after allergen challenge in bronchoalveolar lavage fluid (BALF) and in serum. The relative expression of CC16 and TNF-α mRNA were measured in lung homogenates. A morphometrical analysis of mucus hypersecretion and electron microscopy served to investigate goblet cell metaplasia and Clara cell morphological alterations.</p> <p>Results</p> <p>In non sensitized mice EC-UFP inhalation caused alterations in CC16 concentration, both at protein and mRNA level, and induced Clara cell hyperplasia. In sensitized mice, inhalation of EC-UFP prior to OVA challenge caused most significant alterations of BALF and serum CC16 concentration, BALF total protein and TNF-α relative expression compared to relevant controls; their Clara cells displayed the strongest morphological alterations and strongest goblet cell metaplasia occurred in the small airways. NAC strongly reduced both functional and morphological alterations of Clara cells.</p> <p>Conclusion</p> <p>Our findings demonstrate that oxidative stress plays an important role in EC-UFP-induced augmentation of functional and morphological alterations of Clara cells in allergic lung inflammation.</p>http://www.particleandfibretoxicology.com/content/7/1/11 |
spellingShingle | Schulz Holger Mempel Martin Blume Cornelia Karg Erwin Takenaka Shinji van Miert Erik Weichenmeier Ingrid Alessandrini Francesca Bernard Alfred Behrendt Heidrun Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation Particle and Fibre Toxicology |
title | Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation |
title_full | Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation |
title_fullStr | Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation |
title_full_unstemmed | Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation |
title_short | Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation |
title_sort | effects of ultrafine particles induced oxidative stress on clara cells in allergic lung inflammation |
url | http://www.particleandfibretoxicology.com/content/7/1/11 |
work_keys_str_mv | AT schulzholger effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT mempelmartin effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT blumecornelia effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT kargerwin effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT takenakashinji effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT vanmierterik effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT weichenmeieringrid effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT alessandrinifrancesca effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT bernardalfred effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation AT behrendtheidrun effectsofultrafineparticlesinducedoxidativestressonclaracellsinallergiclunginflammation |