Protocol to purify recombinant inflammatory caspases and assess their catalytic activity in vitro
Summary: The inflammatory caspases, such as caspase-1, caspase-4, or caspase-11, are key enzymes in mammalian innate immunity as they control inflammasome-dependent inflammation. Assessing the specific proteolytic activities of these caspases in the context of a cell remains challenging, which is wh...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-12-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007286 |