Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agent
The probiotic potential of Lactiplantibacillus pentosus CF-6HA isolated from traditionally fermented Aloreña table olives was analyzed in vitro and in silico. Results obtained suggested that this strain can be catalogued as “talented” bacterium exhibiting bacteriocin production with antimicrobial ac...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024044396 |
| _version_ | 1827310661088051200 |
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| author | Hikmate Abriouel Natacha Caballero Gómez Julia Manetsberger Nabil Benomar |
| author_facet | Hikmate Abriouel Natacha Caballero Gómez Julia Manetsberger Nabil Benomar |
| author_sort | Hikmate Abriouel |
| collection | DOAJ |
| description | The probiotic potential of Lactiplantibacillus pentosus CF-6HA isolated from traditionally fermented Aloreña table olives was analyzed in vitro and in silico. Results obtained suggested that this strain can be catalogued as “talented” bacterium exhibiting bacteriocin production with antimicrobial activity against human/animal and plant pathogens, such as Pseudomonas syringae and Verticillium dahliae. The robustness, safety and probiotic potential of L. pentosus CF-6HA was confirmed by in silico analysis. In addition, a plethora of coding genes for defense and adaptability to different life styles besides functional properties were identified. In this sense, defense mechanisms of L. pentosus CF-6HA consist of 17 ISI elements, 98 transposases and 13 temperate phage regions as well as a CRISPR (clustered regularly interspaced short palindromic repeats)/cas system. Moreover, the functionality of this strain was confirmed by the presence of genes coding for secondary metabolites, exopolysaccharides and other bioactive molecules. Finally, we demonstrated the ability of L. pentosus CF-6HA to biotransform selenite to nanoparticles (SeNPs) highlighting its potential role in selenium bioremediation to be exploited in foods, agriculture and the environment; but also for the bio-enrichment of fermented foods with selenium. |
| first_indexed | 2024-04-24T20:03:41Z |
| format | Article |
| id | doaj.art-949ebb69d9fc49ef8dc6d712120cd963 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T20:03:41Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-949ebb69d9fc49ef8dc6d712120cd9632024-03-24T06:59:45ZengElsevierHeliyon2405-84402024-04-01107e28408Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agentHikmate Abriouel0Natacha Caballero Gómez1Julia Manetsberger2Nabil Benomar3Corresponding author. Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Edif. B3, Universidad de Jaén, Campus Las Lagunillas s/n, 23071-Jaén, Spain.; Área de Microbiología, Departamento de Ciencias de La Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071-Jaén, SpainÁrea de Microbiología, Departamento de Ciencias de La Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071-Jaén, SpainÁrea de Microbiología, Departamento de Ciencias de La Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071-Jaén, SpainÁrea de Microbiología, Departamento de Ciencias de La Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071-Jaén, SpainThe probiotic potential of Lactiplantibacillus pentosus CF-6HA isolated from traditionally fermented Aloreña table olives was analyzed in vitro and in silico. Results obtained suggested that this strain can be catalogued as “talented” bacterium exhibiting bacteriocin production with antimicrobial activity against human/animal and plant pathogens, such as Pseudomonas syringae and Verticillium dahliae. The robustness, safety and probiotic potential of L. pentosus CF-6HA was confirmed by in silico analysis. In addition, a plethora of coding genes for defense and adaptability to different life styles besides functional properties were identified. In this sense, defense mechanisms of L. pentosus CF-6HA consist of 17 ISI elements, 98 transposases and 13 temperate phage regions as well as a CRISPR (clustered regularly interspaced short palindromic repeats)/cas system. Moreover, the functionality of this strain was confirmed by the presence of genes coding for secondary metabolites, exopolysaccharides and other bioactive molecules. Finally, we demonstrated the ability of L. pentosus CF-6HA to biotransform selenite to nanoparticles (SeNPs) highlighting its potential role in selenium bioremediation to be exploited in foods, agriculture and the environment; but also for the bio-enrichment of fermented foods with selenium.http://www.sciencedirect.com/science/article/pii/S2405844024044396Aloreña table olivesLactiplantibacillus pentosusIn silico analysisProbioticsFunctional propertiesSelenium |
| spellingShingle | Hikmate Abriouel Natacha Caballero Gómez Julia Manetsberger Nabil Benomar Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agent Heliyon Aloreña table olives Lactiplantibacillus pentosus In silico analysis Probiotics Functional properties Selenium |
| title | Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agent |
| title_full | Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agent |
| title_fullStr | Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agent |
| title_full_unstemmed | Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agent |
| title_short | Dual effects of a bacteriocin-producing Lactiplantibacillus pentosus CF-6HA, isolated from fermented aloreña table olives, as potential probiotic and antimicrobial agent |
| title_sort | dual effects of a bacteriocin producing lactiplantibacillus pentosus cf 6ha isolated from fermented alorena table olives as potential probiotic and antimicrobial agent |
| topic | Aloreña table olives Lactiplantibacillus pentosus In silico analysis Probiotics Functional properties Selenium |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024044396 |
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