Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy)
The characterization of <i>Oenococcus oeni</i> strains isolated from Nero di Troia wine (Apulia, Italy) sampled in two distinct production areas was carried out. The two indigenous populations, consisting of 95 and 97 isolates, displayed high genetic diversity when analyzed by amplified...
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/795 |
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| author | Maria Stella Cappello Vittorio Falco Rosita Curcio Giovanni Mita Giacomo Zapparoli |
| author_facet | Maria Stella Cappello Vittorio Falco Rosita Curcio Giovanni Mita Giacomo Zapparoli |
| author_sort | Maria Stella Cappello |
| collection | DOAJ |
| description | The characterization of <i>Oenococcus oeni</i> strains isolated from Nero di Troia wine (Apulia, Italy) sampled in two distinct production areas was carried out. The two indigenous populations, consisting of 95 and 97 isolates, displayed high genetic diversity when analyzed by amplified fragments length polymorphisms (AFLP). Based on the UPGMA dendrogram obtained by AFLP analysis, the two populations displayed similar genotypes that grouped in the same clusters with a high level of similarity (>95%). One genotype was found in only one of the two areas. Representative strains of each cluster were analyzed for their enzymatic activities (esterase, β-glucosidase, and protease), assayed in whole cells, and tested for their metabolic properties (consumption of L-malic acid, citric acid, acetaldehyde, and arginine) and growth parameters. Significant differences among strains, including the reference strain ATCC BAA-1163, were observed for all of these properties. Principal component analysis evidenced phenotypic differences among strains, and well separated some of them belonging to different genotypes. Strains exhibiting the best performances in most of these traits could be further investigated in order to select possible candidates as malolactic starters for Nero di Troia wine. This study provided insights on the population structure of <i>O. oeni</i> of a local winemaking area useful to the understanding of the regional diversity of this bacterium, an issue not yet completely resolved |
| first_indexed | 2024-03-09T13:16:37Z |
| format | Article |
| id | doaj.art-94cfbf9318bc47c0a313cb983d908441 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:37Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-94cfbf9318bc47c0a313cb983d9084412023-11-30T21:35:33ZengMDPI AGMicroorganisms2076-26072022-04-0110479510.3390/microorganisms10040795Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy)Maria Stella Cappello0Vittorio Falco1Rosita Curcio2Giovanni Mita3Giacomo Zapparoli4CNR, Institute of Science of Food Production (ISPA), Prov.le Lecce-Monteroni, 73100 Lecce, ItalyCNR, Institute of Science of Food Production (ISPA), Prov.le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyCNR, Institute of Science of Food Production (ISPA), Prov.le Lecce-Monteroni, 73100 Lecce, ItalyDepartment of Biotechnology, University of Verona, Strada le Grazie 15, 37134 Verona, ItalyThe characterization of <i>Oenococcus oeni</i> strains isolated from Nero di Troia wine (Apulia, Italy) sampled in two distinct production areas was carried out. The two indigenous populations, consisting of 95 and 97 isolates, displayed high genetic diversity when analyzed by amplified fragments length polymorphisms (AFLP). Based on the UPGMA dendrogram obtained by AFLP analysis, the two populations displayed similar genotypes that grouped in the same clusters with a high level of similarity (>95%). One genotype was found in only one of the two areas. Representative strains of each cluster were analyzed for their enzymatic activities (esterase, β-glucosidase, and protease), assayed in whole cells, and tested for their metabolic properties (consumption of L-malic acid, citric acid, acetaldehyde, and arginine) and growth parameters. Significant differences among strains, including the reference strain ATCC BAA-1163, were observed for all of these properties. Principal component analysis evidenced phenotypic differences among strains, and well separated some of them belonging to different genotypes. Strains exhibiting the best performances in most of these traits could be further investigated in order to select possible candidates as malolactic starters for Nero di Troia wine. This study provided insights on the population structure of <i>O. oeni</i> of a local winemaking area useful to the understanding of the regional diversity of this bacterium, an issue not yet completely resolvedhttps://www.mdpi.com/2076-2607/10/4/795<i>Oenococcus oeni</i>Nero di Troia wineamplified fragments length polymorphismsgenetic diversityenzymatic activitiesmetabolic properties |
| spellingShingle | Maria Stella Cappello Vittorio Falco Rosita Curcio Giovanni Mita Giacomo Zapparoli Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy) Microorganisms <i>Oenococcus oeni</i> Nero di Troia wine amplified fragments length polymorphisms genetic diversity enzymatic activities metabolic properties |
| title | Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy) |
| title_full | Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy) |
| title_fullStr | Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy) |
| title_full_unstemmed | Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy) |
| title_short | Molecular and Physiological Properties of Indigenous Strains of <i>Oenococcus oeni</i> Selected from Nero di Troia Wine (Apulia, Italy) |
| title_sort | molecular and physiological properties of indigenous strains of i oenococcus oeni i selected from nero di troia wine apulia italy |
| topic | <i>Oenococcus oeni</i> Nero di Troia wine amplified fragments length polymorphisms genetic diversity enzymatic activities metabolic properties |
| url | https://www.mdpi.com/2076-2607/10/4/795 |
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