HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular Vesicles
Amyloid beta (Aβ) depositions are more abundant in HIV-infected brains. The blood–brain barrier, with its backbone created by endothelial cells, is assumed to be a core player in Aβ homeostasis and may contribute to Aβ accumulation in the brain. Exposure to HIV increases shedding of extracellular ve...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/8/2741 |
| _version_ | 1797570563127902208 |
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| author | Ibolya E. András Brice B. Sewell Michal Toborek |
| author_facet | Ibolya E. András Brice B. Sewell Michal Toborek |
| author_sort | Ibolya E. András |
| collection | DOAJ |
| description | Amyloid beta (Aβ) depositions are more abundant in HIV-infected brains. The blood–brain barrier, with its backbone created by endothelial cells, is assumed to be a core player in Aβ homeostasis and may contribute to Aβ accumulation in the brain. Exposure to HIV increases shedding of extracellular vesicles (EVs) from human brain endothelial cells and alters EV-Aβ levels. EVs carrying various cargo molecules, including a complex set of proteins, can profoundly affect the biology of surrounding neurovascular unit cells. In the current study, we sought to examine how exposure to HIV, alone or together with Aβ, affects the surface and total proteomic landscape of brain endothelial EVs. By using this unbiased approach, we gained an unprecedented, high-resolution insight into these changes. Our data suggest that HIV and Aβ profoundly remodel the proteome of brain endothelial EVs, altering the pathway networks and functional interactions among proteins. These events may contribute to the EV-mediated amyloid pathology in the HIV-infected brain and may be relevant to HIV-1-associated neurocognitive disorders. |
| first_indexed | 2024-03-10T20:27:17Z |
| format | Article |
| id | doaj.art-94ddc45bf5224744906f8e7bb46d8f9d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:27:17Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-94ddc45bf5224744906f8e7bb46d8f9d2023-11-19T21:42:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218274110.3390/ijms21082741HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular VesiclesIbolya E. András0Brice B. Sewell1Michal Toborek2Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33136-1019, USADepartment of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33136-1019, USADepartment of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33136-1019, USAAmyloid beta (Aβ) depositions are more abundant in HIV-infected brains. The blood–brain barrier, with its backbone created by endothelial cells, is assumed to be a core player in Aβ homeostasis and may contribute to Aβ accumulation in the brain. Exposure to HIV increases shedding of extracellular vesicles (EVs) from human brain endothelial cells and alters EV-Aβ levels. EVs carrying various cargo molecules, including a complex set of proteins, can profoundly affect the biology of surrounding neurovascular unit cells. In the current study, we sought to examine how exposure to HIV, alone or together with Aβ, affects the surface and total proteomic landscape of brain endothelial EVs. By using this unbiased approach, we gained an unprecedented, high-resolution insight into these changes. Our data suggest that HIV and Aβ profoundly remodel the proteome of brain endothelial EVs, altering the pathway networks and functional interactions among proteins. These events may contribute to the EV-mediated amyloid pathology in the HIV-infected brain and may be relevant to HIV-1-associated neurocognitive disorders.https://www.mdpi.com/1422-0067/21/8/2741HIV-1amyloid betaextracellular vesiclesblood–brain barrier |
| spellingShingle | Ibolya E. András Brice B. Sewell Michal Toborek HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular Vesicles International Journal of Molecular Sciences HIV-1 amyloid beta extracellular vesicles blood–brain barrier |
| title | HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular Vesicles |
| title_full | HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular Vesicles |
| title_fullStr | HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular Vesicles |
| title_full_unstemmed | HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular Vesicles |
| title_short | HIV-1 and Amyloid Beta Remodel Proteome of Brain Endothelial Extracellular Vesicles |
| title_sort | hiv 1 and amyloid beta remodel proteome of brain endothelial extracellular vesicles |
| topic | HIV-1 amyloid beta extracellular vesicles blood–brain barrier |
| url | https://www.mdpi.com/1422-0067/21/8/2741 |
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