A novel approach for measuring sphingosine-1-phosphate and lysophosphatidic acid binding to carrier proteins using monoclonal antibodies and the Kinetic Exclusion Assay

A novel approach for measuring sphingosine-1-phosphate and lysophosphatidic acid binding to carrier proteins using monoclonal antibodies and the Kinetic Exclusion Assay

Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are bioactive signaling lysophospholipids that activate specific G protein-coupled receptors on the cell surface triggering numerous biological events. In circulation, S1P and LPA associate with specific carrier proteins or chaperones; se...

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Bibliographic Details
Main Authors: Jonathan K. Fleming, Thomas R. Glass, Steve J. Lackie, Jonathan M. Wojciak
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Journal of Lipid Research
Subjects:
anti-lipid antibody
apolipoproteins
Kinetic Exclusion Assay
lipids
competitive affinity analysis
lipoproteins
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520309408

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http://www.sciencedirect.com/science/article/pii/S0022227520309408

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