Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells

The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [Kilchert et al., 2016]). The following protocol describes an approach...

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Bibliographic Details
Main Authors: Michal Domanski, John LaCava
Format: Article
Language:English
Published: Bio-protocol LLC 2017-04-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=2238&type=0