Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration
Abstract Reconstruction of complex cartilage defects has remained a great challenge for tissue engineering due to the lack of stem cells and chronic inflammation within the joint. In this study, we have developed an injectable pig cartilage-derived decellularized extracellular matrix (dECM) hydrogel...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | npj Regenerative Medicine |
| Online Access: | https://doi.org/10.1038/s41536-022-00269-w |
| _version_ | 1797977549596262400 |
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| author | Junfeng Zeng Liping Huang Huazhang Xiong Qianjin Li Chenyu Wu Yizhou Huang Huiqi Xie Bin Shen |
| author_facet | Junfeng Zeng Liping Huang Huazhang Xiong Qianjin Li Chenyu Wu Yizhou Huang Huiqi Xie Bin Shen |
| author_sort | Junfeng Zeng |
| collection | DOAJ |
| description | Abstract Reconstruction of complex cartilage defects has remained a great challenge for tissue engineering due to the lack of stem cells and chronic inflammation within the joint. In this study, we have developed an injectable pig cartilage-derived decellularized extracellular matrix (dECM) hydrogels for the repair of cartilage defects, which has shown sound biocompatibility and immunomodulatory capacity both in vitro and in vivo. The dECM hydrogels can enhance the chondrogenic differentiation of human urine-derived stem cells (USCs). As shown by in vitro experiment, the USCs in the dECM hydrogels have survived, proliferated, and produced a mass of cartilage-specific extracellular matrix containing collagen II and aggrecan. And the USCs-laden dECM hydrogels have shown the capacity to promote the secretion of extracellular matrix, modulate the immune response and promote cartilage regeneration in the rat model for cartilage defect. |
| first_indexed | 2024-04-11T05:09:50Z |
| format | Article |
| id | doaj.art-951e9fac9b674febb61085cd582bb964 |
| institution | Directory Open Access Journal |
| issn | 2057-3995 |
| language | English |
| last_indexed | 2024-04-11T05:09:50Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Regenerative Medicine |
| spelling | doaj.art-951e9fac9b674febb61085cd582bb9642022-12-25T12:07:41ZengNature Portfolionpj Regenerative Medicine2057-39952022-12-017111210.1038/s41536-022-00269-wInjectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regenerationJunfeng Zeng0Liping Huang1Huazhang Xiong2Qianjin Li3Chenyu Wu4Yizhou Huang5Huiqi Xie6Bin Shen7Orthopedics Research Institute, Department of Orthopedics, West China Hospital, Sichuan UniversityLaboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, Med-X Center for Materials, State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityThe First Department of Orthopedics, Affiliated Hospital of Zunyi Medical UniversityLaboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, Med-X Center for Materials, State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityLaboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, Med-X Center for Materials, State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityLaboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, Med-X Center for Materials, State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityLaboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, Med-X Center for Materials, State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityOrthopedics Research Institute, Department of Orthopedics, West China Hospital, Sichuan UniversityAbstract Reconstruction of complex cartilage defects has remained a great challenge for tissue engineering due to the lack of stem cells and chronic inflammation within the joint. In this study, we have developed an injectable pig cartilage-derived decellularized extracellular matrix (dECM) hydrogels for the repair of cartilage defects, which has shown sound biocompatibility and immunomodulatory capacity both in vitro and in vivo. The dECM hydrogels can enhance the chondrogenic differentiation of human urine-derived stem cells (USCs). As shown by in vitro experiment, the USCs in the dECM hydrogels have survived, proliferated, and produced a mass of cartilage-specific extracellular matrix containing collagen II and aggrecan. And the USCs-laden dECM hydrogels have shown the capacity to promote the secretion of extracellular matrix, modulate the immune response and promote cartilage regeneration in the rat model for cartilage defect.https://doi.org/10.1038/s41536-022-00269-w |
| spellingShingle | Junfeng Zeng Liping Huang Huazhang Xiong Qianjin Li Chenyu Wu Yizhou Huang Huiqi Xie Bin Shen Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration npj Regenerative Medicine |
| title | Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration |
| title_full | Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration |
| title_fullStr | Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration |
| title_full_unstemmed | Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration |
| title_short | Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration |
| title_sort | injectable decellularized cartilage matrix hydrogel encapsulating urine derived stem cells for immunomodulatory and cartilage defect regeneration |
| url | https://doi.org/10.1038/s41536-022-00269-w |
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