Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats
Although autogenous nerve transplantation is the gold standard for treating peripheral nerve defects of considerable length, it still has some shortcomings, such as insufficient donors and secondary injury. Composite chitosan scaffolds loaded with controlled release of nerve growth factor can promot...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
|
| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=5;spage=1146;epage=1155;aulast=Liu |
| _version_ | 1819041048345706496 |
|---|---|
| author | Fa-Dong Liu Hong-Mei Duan Fei Hao Wen Zhao Yu-Dan Gao Peng Hao Zhao-Yang Yang Xiao-Guang Li |
| author_facet | Fa-Dong Liu Hong-Mei Duan Fei Hao Wen Zhao Yu-Dan Gao Peng Hao Zhao-Yang Yang Xiao-Guang Li |
| author_sort | Fa-Dong Liu |
| collection | DOAJ |
| description | Although autogenous nerve transplantation is the gold standard for treating peripheral nerve defects of considerable length, it still has some shortcomings, such as insufficient donors and secondary injury. Composite chitosan scaffolds loaded with controlled release of nerve growth factor can promote neuronal survival and axonal regeneration after short-segment sciatic nerve defects. However, the effects on extended nerve defects remain poorly understood. In this study, we used chitosan scaffolds loaded with nerve growth factor for 8 weeks to repair long-segment (20 mm) sciatic nerve defects in adult rats. The results showed that treatment markedly promoted the recovery of motor and sensory functions. The regenerated sciatic nerve not only reconnected with neurons but neural circuits with the central nervous system were also reconstructed. In addition, the regenerated sciatic nerve reconnected the motor endplate with the target muscle. Therefore, this novel biomimetic scaffold can promote the regeneration of extended sciatic nerve defects and reconstruct functional circuits. This provides a promising method for the clinical treatment of extended peripheral nerve injury. This study was approved by the Animal Ethics Committee of Capital Medical University, China (approval No. AEEI-2017-033) on March 21, 2017. |
| first_indexed | 2024-12-21T09:18:48Z |
| format | Article |
| id | doaj.art-95218e85a35f4b9ca40ac24b0a4a2801 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-21T09:18:48Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-95218e85a35f4b9ca40ac24b0a4a28012022-12-21T19:09:04ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-011751146115510.4103/1673-5374.324860Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in ratsFa-Dong LiuHong-Mei DuanFei HaoWen ZhaoYu-Dan GaoPeng HaoZhao-Yang YangXiao-Guang LiAlthough autogenous nerve transplantation is the gold standard for treating peripheral nerve defects of considerable length, it still has some shortcomings, such as insufficient donors and secondary injury. Composite chitosan scaffolds loaded with controlled release of nerve growth factor can promote neuronal survival and axonal regeneration after short-segment sciatic nerve defects. However, the effects on extended nerve defects remain poorly understood. In this study, we used chitosan scaffolds loaded with nerve growth factor for 8 weeks to repair long-segment (20 mm) sciatic nerve defects in adult rats. The results showed that treatment markedly promoted the recovery of motor and sensory functions. The regenerated sciatic nerve not only reconnected with neurons but neural circuits with the central nervous system were also reconstructed. In addition, the regenerated sciatic nerve reconnected the motor endplate with the target muscle. Therefore, this novel biomimetic scaffold can promote the regeneration of extended sciatic nerve defects and reconstruct functional circuits. This provides a promising method for the clinical treatment of extended peripheral nerve injury. This study was approved by the Animal Ethics Committee of Capital Medical University, China (approval No. AEEI-2017-033) on March 21, 2017.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=5;spage=1146;epage=1155;aulast=Liuaxon; chitosan; functional recovery; myelin sheath; nerve growth factor; peripheral nerve injury; pseudorabies virus; regeneration; scaffold; sciatic nerve |
| spellingShingle | Fa-Dong Liu Hong-Mei Duan Fei Hao Wen Zhao Yu-Dan Gao Peng Hao Zhao-Yang Yang Xiao-Guang Li Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats Neural Regeneration Research axon; chitosan; functional recovery; myelin sheath; nerve growth factor; peripheral nerve injury; pseudorabies virus; regeneration; scaffold; sciatic nerve |
| title | Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats |
| title_full | Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats |
| title_fullStr | Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats |
| title_full_unstemmed | Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats |
| title_short | Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats |
| title_sort | biomimetic chitosan scaffolds with long term controlled release of nerve growth factor repairs 20 mm long sciatic nerve defects in rats |
| topic | axon; chitosan; functional recovery; myelin sheath; nerve growth factor; peripheral nerve injury; pseudorabies virus; regeneration; scaffold; sciatic nerve |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=5;spage=1146;epage=1155;aulast=Liu |
| work_keys_str_mv | AT fadongliu biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats AT hongmeiduan biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats AT feihao biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats AT wenzhao biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats AT yudangao biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats AT penghao biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats AT zhaoyangyang biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats AT xiaoguangli biomimeticchitosanscaffoldswithlongtermcontrolledreleaseofnervegrowthfactorrepairs20mmlongsciaticnervedefectsinrats |