Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase
<p>Abstract</p> <p>Background</p> <p>The subcloning of a DNA fragment from an entry vector into a destination vector is a routinely performed task in molecular biology labs.</p> <p>Results</p> <p>We here present a novel benchtop procedure to achi...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
BMC
2007-11-01
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Series: | Journal of Biological Engineering |
Online Access: | http://www.jbioleng.org/content/1/1/7 |