Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase

<p>Abstract</p> <p>Background</p> <p>The subcloning of a DNA fragment from an entry vector into a destination vector is a routinely performed task in molecular biology labs.</p> <p>Results</p> <p>We here present a novel benchtop procedure to achi...

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Bibliographic Details
Main Authors: Klingenspor Martin, Fromme Tobias
Format: Article
Language:English
Published: BMC 2007-11-01
Series:Journal of Biological Engineering
Online Access:http://www.jbioleng.org/content/1/1/7