Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications

Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications

Chinese hamster ovary (CHO) cells are the most important platform for producing biotherapeutics. Random integration of a transgene into epigenetically instable regions of the genome results in silencing of the gene of interest and loss of productivity during upstream processing. Therefore, cost- and...

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Bibliographic Details
Main Authors: Oliver Hertel, Anne Neuss, Tobias Busche, David Brandt, Jörn Kalinowski, Janina Bahnemann, Thomas Noll
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
CRISPR/Cas9
CHO
cell line development
ChIP-seq
epigenetics
safe harbor
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.1010719/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2022.1010719/full

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