A ten-year search for synchronous cells: obstacles, solutions and practical applications

My effort to use synchronously dividing cultures to examine the Escherichia coli cell cycle involved a ten-year struggle with failure after failure punctuated by a few gratifying successes, especially at the end. In this essay, I recount my personal journey in this obsessive experimental pursuit....

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Bibliographic Details
Main Author: Charles Edward Helmstetter
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-03-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00238/full