Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction.
Serum and glucocorticoid inducible kinase 1 (SGK1) plays a pivotal role in early angiogenesis during embryonic development. In this study, we sought to define the SGK1 downstream signalling pathways in the adult heart and to elucidate their role in cardiac neo-angiogenesis and wound healing after my...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3827188?pdf=render |
| _version_ | 1811320774886162432 |
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| author | Elham Zarrinpashneh Tommaso Poggioli Padmini Sarathchandra Jonas Lexow Laurent Monassier Cesare Terracciano Florian Lang Federico Damilano Jessica Q Zhou Anthony Rosenzweig Nadia Rosenthal Maria Paola Santini |
| author_facet | Elham Zarrinpashneh Tommaso Poggioli Padmini Sarathchandra Jonas Lexow Laurent Monassier Cesare Terracciano Florian Lang Federico Damilano Jessica Q Zhou Anthony Rosenzweig Nadia Rosenthal Maria Paola Santini |
| author_sort | Elham Zarrinpashneh |
| collection | DOAJ |
| description | Serum and glucocorticoid inducible kinase 1 (SGK1) plays a pivotal role in early angiogenesis during embryonic development. In this study, we sought to define the SGK1 downstream signalling pathways in the adult heart and to elucidate their role in cardiac neo-angiogenesis and wound healing after myocardial ischemia. To this end, we employed a viable SGK1 knockout mouse model generated in a 129/SvJ background. Ablation of SGK1 in these mice caused a significant decrease in phosphorylation of SGK1 target protein NDRG1, which correlated with alterations in NF-κB signalling and expression of its downstream target protein, VEGF-A. Disruption of these signalling pathways was accompanied by smaller heart and body size. Moreover, the lack of SGK1 led to defective endothelial cell (ECs) migration and tube formation in vitro, and increased scarring with decreased angiogenesis in vivo after myocardial infarct. This study underscores the importance of SGK1 signalling in cardiac neo-angiogenesis and wound healing after an ischemic insult in vivo. |
| first_indexed | 2024-04-13T13:05:20Z |
| format | Article |
| id | doaj.art-956b000831b64fd4b0ff71dbce75f1f2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T13:05:20Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-956b000831b64fd4b0ff71dbce75f1f22022-12-22T02:45:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e8026810.1371/journal.pone.0080268Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction.Elham ZarrinpashnehTommaso PoggioliPadmini SarathchandraJonas LexowLaurent MonassierCesare TerraccianoFlorian LangFederico DamilanoJessica Q ZhouAnthony RosenzweigNadia RosenthalMaria Paola SantiniSerum and glucocorticoid inducible kinase 1 (SGK1) plays a pivotal role in early angiogenesis during embryonic development. In this study, we sought to define the SGK1 downstream signalling pathways in the adult heart and to elucidate their role in cardiac neo-angiogenesis and wound healing after myocardial ischemia. To this end, we employed a viable SGK1 knockout mouse model generated in a 129/SvJ background. Ablation of SGK1 in these mice caused a significant decrease in phosphorylation of SGK1 target protein NDRG1, which correlated with alterations in NF-κB signalling and expression of its downstream target protein, VEGF-A. Disruption of these signalling pathways was accompanied by smaller heart and body size. Moreover, the lack of SGK1 led to defective endothelial cell (ECs) migration and tube formation in vitro, and increased scarring with decreased angiogenesis in vivo after myocardial infarct. This study underscores the importance of SGK1 signalling in cardiac neo-angiogenesis and wound healing after an ischemic insult in vivo.http://europepmc.org/articles/PMC3827188?pdf=render |
| spellingShingle | Elham Zarrinpashneh Tommaso Poggioli Padmini Sarathchandra Jonas Lexow Laurent Monassier Cesare Terracciano Florian Lang Federico Damilano Jessica Q Zhou Anthony Rosenzweig Nadia Rosenthal Maria Paola Santini Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction. PLoS ONE |
| title | Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction. |
| title_full | Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction. |
| title_fullStr | Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction. |
| title_full_unstemmed | Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction. |
| title_short | Ablation of SGK1 impairs endothelial cell migration and tube formation leading to decreased neo-angiogenesis following myocardial infarction. |
| title_sort | ablation of sgk1 impairs endothelial cell migration and tube formation leading to decreased neo angiogenesis following myocardial infarction |
| url | http://europepmc.org/articles/PMC3827188?pdf=render |
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