Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs
The effect of growth temperature on glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts was firstly evaluated, and then their distributions in the cotyledon, hypocotyl and root at the optimum temperature were investigated. The profiles of isothiocyanates and nitrile i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-07-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614001480 |
| _version_ | 1823943146213474304 |
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| author | Liping Guo Runqiang Yang Zhiying Wang Qianghui Guo Zhenxin Gu |
| author_facet | Liping Guo Runqiang Yang Zhiying Wang Qianghui Guo Zhenxin Gu |
| author_sort | Liping Guo |
| collection | DOAJ |
| description | The effect of growth temperature on glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts was firstly evaluated, and then their distributions in the cotyledon, hypocotyl and root at the optimum temperature were investigated. The profiles of isothiocyanates and nitrile in seeds, sprouts and different organs were also analyzed. Glucoraphanin content and sulforaphane formation declined with sprouts growth. Myrosinase activity in sprouts, stable for the first 3 days of germination, slowly increased threefold by day 7. The highest levels of glucoraphanin and sulforaphane were recorded in non-germinated seeds. Sprouts grown at 25 °C had higher glucoraphanin content and sulforaphane formation than that grown at 20 and 30 °C. In addition, cotyledon had significantly higher glucoraphanin content and sulforaphane formation than hypocotyl and root. Glucoraphanin and sulforaphane were barely detectable in root, despite its high myrosinase activity. The most abundant hydrolysis product in seeds and sprouts, cotyledon and hypocotyl was sulforaphane. |
| first_indexed | 2024-12-17T04:46:58Z |
| format | Article |
| id | doaj.art-9572a9b2c25d4114a9a50366226e8413 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T04:46:58Z |
| publishDate | 2014-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-9572a9b2c25d4114a9a50366226e84132022-12-21T22:03:03ZengElsevierJournal of Functional Foods1756-46462014-07-0197077Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organsLiping Guo0Runqiang Yang1Zhiying Wang2Qianghui Guo3Zhenxin Gu4College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China; College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China; Corresponding author. Tel.: +86 025 84396293; fax: +86 025 84396293.The effect of growth temperature on glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts was firstly evaluated, and then their distributions in the cotyledon, hypocotyl and root at the optimum temperature were investigated. The profiles of isothiocyanates and nitrile in seeds, sprouts and different organs were also analyzed. Glucoraphanin content and sulforaphane formation declined with sprouts growth. Myrosinase activity in sprouts, stable for the first 3 days of germination, slowly increased threefold by day 7. The highest levels of glucoraphanin and sulforaphane were recorded in non-germinated seeds. Sprouts grown at 25 °C had higher glucoraphanin content and sulforaphane formation than that grown at 20 and 30 °C. In addition, cotyledon had significantly higher glucoraphanin content and sulforaphane formation than hypocotyl and root. Glucoraphanin and sulforaphane were barely detectable in root, despite its high myrosinase activity. The most abundant hydrolysis product in seeds and sprouts, cotyledon and hypocotyl was sulforaphane.http://www.sciencedirect.com/science/article/pii/S1756464614001480GlucoraphaninSulforaphaneMyrosinaseBroccoli sproutsOrganGermination |
| spellingShingle | Liping Guo Runqiang Yang Zhiying Wang Qianghui Guo Zhenxin Gu Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs Journal of Functional Foods Glucoraphanin Sulforaphane Myrosinase Broccoli sprouts Organ Germination |
| title | Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs |
| title_full | Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs |
| title_fullStr | Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs |
| title_full_unstemmed | Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs |
| title_short | Glucoraphanin, sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs |
| title_sort | glucoraphanin sulforaphane and myrosinase activity in germinating broccoli sprouts as affected by growth temperature and plant organs |
| topic | Glucoraphanin Sulforaphane Myrosinase Broccoli sprouts Organ Germination |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614001480 |
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