Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system

Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system

Summary: In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D cultur...

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Bibliographic Details
Main Authors: Daniela Belloni, Marina Ferrarini, Elisabetta Ferrero, Virginia Guzzeloni, Federica Barbaglio, Paolo Ghia, Cristina Scielzo
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cancer
Immunology
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004816

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722004816

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