Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system
Summary: In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D cultur...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722004816 |
| _version_ | 1811310003091406848 |
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| author | Daniela Belloni Marina Ferrarini Elisabetta Ferrero Virginia Guzzeloni Federica Barbaglio Paolo Ghia Cristina Scielzo |
| author_facet | Daniela Belloni Marina Ferrarini Elisabetta Ferrero Virginia Guzzeloni Federica Barbaglio Paolo Ghia Cristina Scielzo |
| author_sort | Daniela Belloni |
| collection | DOAJ |
| description | Summary: In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can efficiently recapitulate tumor-stroma crosstalk and allows the testing of drugs such as ibrutinib and bortezomib. Moreover, this protocol can be used for the generation of other and more complex tumor microenvironments.For complete details on the use and execution of this protocol, please refer to Belloni et al. (2018) and Barbaglio et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T09:51:07Z |
| format | Article |
| id | doaj.art-95976befc988423dabe8ce495c828bd6 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T09:51:07Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-95976befc988423dabe8ce495c828bd62022-12-22T02:51:35ZengElsevierSTAR Protocols2666-16672022-09-0133101601Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture systemDaniela Belloni0Marina Ferrarini1Elisabetta Ferrero2Virginia Guzzeloni3Federica Barbaglio4Paolo Ghia5Cristina Scielzo6B Cell Neoplasia Unit, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, Italy; Università Vita Salute San Raffaele, 20123 Milan, Italy; Unit of Malignant B Cells Biology and 3D Modelling, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, ItalyB Cell Neoplasia Unit, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, ItalyB Cell Neoplasia Unit, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, ItalyB Cell Neoplasia Unit, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, ItalyUnit of Malignant B Cells Biology and 3D Modelling, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, ItalyB Cell Neoplasia Unit, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, Italy; Università Vita Salute San Raffaele, 20123 Milan, Italy; Strategic Research Program on CLL, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, ItalyUnit of Malignant B Cells Biology and 3D Modelling, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20123 Milan, Italy; Corresponding authorSummary: In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D culture can efficiently recapitulate tumor-stroma crosstalk and allows the testing of drugs such as ibrutinib and bortezomib. Moreover, this protocol can be used for the generation of other and more complex tumor microenvironments.For complete details on the use and execution of this protocol, please refer to Belloni et al. (2018) and Barbaglio et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722004816Cell BiologyCell cultureCancerImmunologyBiotechnology and bioengineering |
| spellingShingle | Daniela Belloni Marina Ferrarini Elisabetta Ferrero Virginia Guzzeloni Federica Barbaglio Paolo Ghia Cristina Scielzo Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system STAR Protocols Cell Biology Cell culture Cancer Immunology Biotechnology and bioengineering |
| title | Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system |
| title_full | Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system |
| title_fullStr | Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system |
| title_full_unstemmed | Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system |
| title_short | Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system |
| title_sort | protocol for generation of 3d bone marrow surrogate microenvironments in a rotary cell culture system |
| topic | Cell Biology Cell culture Cancer Immunology Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722004816 |
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