Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system

Protocol for generation of 3D bone marrow surrogate microenvironments in a rotary cell culture system

Summary: In this protocol, we describe how to generate 3D culture surrogates of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) bone marrow microenvironments. We detail the use of culturing scaffolds populated with BM stromal cells and tumor cells in the RCCS™ bioreactor. This 3D cultur...

Full description

Bibliographic Details
Main Authors:	Daniela Belloni, Marina Ferrarini, Elisabetta Ferrero, Virginia Guzzeloni, Federica Barbaglio, Paolo Ghia, Cristina Scielzo
Format:	Article
Language:	English
Published:	Elsevier 2022-09-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell culture Cancer Immunology Biotechnology and bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722004816

Similar Items

Protocol for ex vivo culture of patient-derived tumor fragments
by: Lisanne M. Roelofsen, et al.
Published: (2023-06-01)

Probing human immune cell mechanics using acoustic force spectroscopy
by: Tom M.J. Evers, et al.
Published: (2024-03-01)

Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
by: Mehmet Emrah Selli, et al.
Published: (2023-03-01)

Transwell migration assay to interrogate human CAR-T cell chemotaxis
by: Arman Oner, et al.
Published: (2022-12-01)

Purification of immune-active macrophage super enhancers by chemical cross-linked chromatin immune precipitation
by: Sonali Das, et al.
Published: (2023-03-01)

Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured human cells and mouse neurons
by: Takashi Kanadome, et al.
Published: (2024-03-01)

Nanoscale organization of the endogenous ASC speck
by: Ivo M. Glück, et al.
Published: (2023-12-01)

Protocol to engineer apical-out airway organoids using suspension culture of human airway basal stem cell aggregates
by: Piyumi Wijesekara, et al.
Published: (2023-06-01)

Calcium measurements in enzymatically dissociated or mechanically microdissected mouse primary skeletal muscle fibers
by: Sonia Youhanna, et al.
Published: (2023-06-01)

Protocol for efficient human MSC chondrogenesis via Wnt antagonism instead of TGF-β
by: Chen-Chan Hsieh, et al.
Published: (2023-12-01)

Analysis of mitochondrial double-stranded RNAs in human cells
by: Sujin Kim, et al.
Published: (2023-03-01)

Self-assembly of polyphosphazene immunoadjuvant with poly(ethylene oxide) enables advanced nanoscale delivery modalities and regulated pH-dependent cellular membrane activity
by: Alexander K. Andrianov, et al.
Published: (2016-04-01)

Protocol for mathematical prediction of patient response and survival to immune checkpoint inhibitor immunotherapy
by: Joseph D. Butner, et al.
Published: (2022-12-01)

Protocol to generate an in vitro model to study vascular calcification using human endothelial and smooth muscle cells
by: Elisa Persiani, et al.
Published: (2023-06-01)

Protocol for observing microtubules and microtubule ends in both fixed and live primary microglia cells
by: Caterina Sanchini, et al.
Published: (2023-09-01)

Protocol to generate and utilize pancreatic tissue slices to study endocrine and exocrine physiology in situ from mouse and human tissue
by: Julia K. Panzer, et al.
Published: (2023-09-01)

A multi-well bioreactor for cartilage tissue engineering experiments
by: Yann D. Ladner, et al.
Published: (2023-07-01)

Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
by: Luisa Blöbaum, et al.
Published: (2023-09-01)

Chemical biotechnology and bioengineering /
by: Qian, Xuhong, author, et al.
Published: (2015)

Career development in bioengineering and biotechnology /
by: Madhavan, Guruprasad, et al.
Published: (2008)

Biotechnology and Bioengineering /
by: Jacob-Lopes, Eduardo, editor, et al.
Published: (2019)

Essence and Applications of Biotechnology /
by: Spencer, Valentine, author 652547, et al.
Published: (2012)

Advanced Biotechnology and its Products /
by: Lacey, Rosalia, author 644550, et al.
Published: (2012)

Biotechnology and bioengineering
Published: (1950)

Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors
by: Samuel D. Pollock, et al.
Published: (2023-12-01)

A protocol for high-throughput screening for immunomodulatory compounds using human primary cells
by: Katherine Chew, et al.
Published: (2023-09-01)

Protocol to encapsulate cerebral organoids with alginate hydrogel shell to induce volumetric compression
by: Zitian Wang, et al.
Published: (2024-06-01)

Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment
by: Junjun Li, et al.
Published: (2024-02-01)

Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates
by: Sejin Bae, et al.
Published: (2021-02-01)

A co-culture model system to quantify antibody-dependent cellular cytotoxicity in human breast cancer cells using an engineered natural killer cell line
by: Roos Vincken, et al.
Published: (2023-06-01)

Protocol for generating postganglionic sympathetic neurons using human pluripotent stem cells for electrophysiological and functional assessments
by: Hsueh-Fu Wu, et al.
Published: (2024-06-01)

Protocol for purification of cells in their native state using reversible aptamer-antidote pairs
by: Martin D. Requena, et al.
Published: (2023-09-01)

Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
by: Zitong Yu, et al.
Published: (2023-03-01)

Protocol for electrical modulation of ERK signaling dynamics in cell lines
by: Kan Zhu, et al.
Published: (2022-12-01)

Second conference on industrial immunology, 4-5 July 1994, Brighton, UK / [proceedings]
by: Institution of Chemical Engineers (Great Britain), et al.
Published: (1994)

Biotechnology and bioengineering /
by: Flynne, William G.
Published: (2008)

Current Developments in Biotechnology and Bioengineering : Functional Genomics and Metabolic Engineering /
by: Gunasekaran, Paramasamy, editors, et al.
Published: (2016)

History and trends in bioprocessing and biotransformation /
by: Scheper, T., et al.
Published: (2002)

Handbook of Biotechnology and Biological Engineering /
by: Lacey, Rosalia, author 644550, et al.
Published: (2012)

Biology for engineers /
by: Johnson, Arthur T. (Arthur Thomas), 1941-
Published: (2011)