Conserved PCR primer set designing for closely-related species to complete mitochondrial genome sequencing using a sliding window-based PSO algorithm.

BACKGROUND: Complete mitochondrial (mt) genome sequencing is becoming increasingly common for phylogenetic reconstruction and as a model for genome evolution. For long template sequencing, i.e., like the entire mtDNA, it is essential to design primers for Polymerase Chain Reaction (PCR) amplicons wh...

Full description

Bibliographic Details
Main Authors: Cheng-Hong Yang, Hsueh-Wei Chang, Chang-Hsuan Ho, Yii-Cheng Chou, Li-Yeh Chuang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3060828?pdf=render