Enzymatic Construction of Protein Polymer/Polyprotein Using OaAEP1 and TEV Protease

The development of chemical and biological coupling technologies in recent years has made possible of protein polymers engineering. We have developed an enzymatic method for building polyproteins using a protein ligase OaAEP1 (asparagine endopeptidase 1) and protease TEV (tobacco etching virus). Usi...

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Bibliographic Details
Main Authors: Yibing Deng, Shengchao Shi, Bin Zheng, Tao Wu, Peng zheng
Format: Article
Language:English
Published: Bio-protocol LLC 2020-04-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=3596&type=0