Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9

Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9

OCT4 is a crucial transcription factor in the pluripotent stem cell gene regulatory network and an essential factor for pluripotent reprogramming. We engineered the previously reported HEL24.3 hiPSC to generate an OCT4 reporter cell line by knocking-in a T2A nuclear EmGFP reporter cassette before th...

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Bibliographic Details
Main Authors: Diego Balboa, Jere Weltner, Yuval Novik, Solja Eurola, Kirmo Wartiovaara, Timo Otonkoski
Format: Article
Language:English
Published: Elsevier 2017-08-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506117301307

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http://www.sciencedirect.com/science/article/pii/S1873506117301307

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