Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory Evolution
Docosahexaenoic acid (DHA, 22:6n-3) plays significant roles in enhancing human health and preventing human diseases. The heterotrophic marine dinoflagellate Crypthecodinium cohnii is a good candidate to produce high-quality DHA. To overcome the inhibition caused by the fermentation supernatant in th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.824189/full |
| _version_ | 1818501015518838784 |
|---|---|
| author | Liangsen Liu Liangsen Liu Liangsen Liu Jinjin Diao Jinjin Diao Jinjin Diao Yali Bi Yali Bi Yali Bi Lei Zeng Lei Zeng Lei Zeng Fangzhong Wang Fangzhong Wang Fangzhong Wang Fangzhong Wang Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang |
| author_facet | Liangsen Liu Liangsen Liu Liangsen Liu Jinjin Diao Jinjin Diao Jinjin Diao Yali Bi Yali Bi Yali Bi Lei Zeng Lei Zeng Lei Zeng Fangzhong Wang Fangzhong Wang Fangzhong Wang Fangzhong Wang Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang |
| author_sort | Liangsen Liu |
| collection | DOAJ |
| description | Docosahexaenoic acid (DHA, 22:6n-3) plays significant roles in enhancing human health and preventing human diseases. The heterotrophic marine dinoflagellate Crypthecodinium cohnii is a good candidate to produce high-quality DHA. To overcome the inhibition caused by the fermentation supernatant in the late fermentation stage of DHA-producing C. cohnii, fermentation supernatant-based adaptive laboratory evolution (FS-ALE) was conducted. The cell growth and DHA productivity of the evolved strain (FS280) obtained after 280 adaptive cycles corresponding to 840 days of evolution were increased by 161.87 and 311.23%, respectively, at 72 h under stress conditions and increased by 19.87 and 51.79% without any stress compared with the starting strain, demonstrating the effectiveness of FS-ALE. In addition, a comparative proteomic analysis identified 11,106 proteins and 910 differentially expressed proteins, including six stress-responsive proteins, as well as the up- and downregulated pathways in FS280 that might contribute to its improved cell growth and DHA accumulation. Our study demonstrated that FS-ALE could be a valuable solution to relieve the inhibition of the fermentation supernatant at the late stage of normal fermentation of heterotrophic microalgae. |
| first_indexed | 2024-12-10T20:50:27Z |
| format | Article |
| id | doaj.art-95c2e4a944b64b8984b995901c3aea3d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T20:50:27Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-95c2e4a944b64b8984b995901c3aea3d2022-12-22T01:34:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-03-011310.3389/fmicb.2022.824189824189Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory EvolutionLiangsen Liu0Liangsen Liu1Liangsen Liu2Jinjin Diao3Jinjin Diao4Jinjin Diao5Yali Bi6Yali Bi7Yali Bi8Lei Zeng9Lei Zeng10Lei Zeng11Fangzhong Wang12Fangzhong Wang13Fangzhong Wang14Fangzhong Wang15Lei Chen16Lei Chen17Lei Chen18Weiwen Zhang19Weiwen Zhang20Weiwen Zhang21Weiwen Zhang22Weiwen Zhang23Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCenter for Biosafety Research and Strategy, Tianjin University, Tianjin, ChinaLaw School, Tianjin University, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaCenter for Biosafety Research and Strategy, Tianjin University, Tianjin, ChinaLaw School, Tianjin University, Tianjin, ChinaDocosahexaenoic acid (DHA, 22:6n-3) plays significant roles in enhancing human health and preventing human diseases. The heterotrophic marine dinoflagellate Crypthecodinium cohnii is a good candidate to produce high-quality DHA. To overcome the inhibition caused by the fermentation supernatant in the late fermentation stage of DHA-producing C. cohnii, fermentation supernatant-based adaptive laboratory evolution (FS-ALE) was conducted. The cell growth and DHA productivity of the evolved strain (FS280) obtained after 280 adaptive cycles corresponding to 840 days of evolution were increased by 161.87 and 311.23%, respectively, at 72 h under stress conditions and increased by 19.87 and 51.79% without any stress compared with the starting strain, demonstrating the effectiveness of FS-ALE. In addition, a comparative proteomic analysis identified 11,106 proteins and 910 differentially expressed proteins, including six stress-responsive proteins, as well as the up- and downregulated pathways in FS280 that might contribute to its improved cell growth and DHA accumulation. Our study demonstrated that FS-ALE could be a valuable solution to relieve the inhibition of the fermentation supernatant at the late stage of normal fermentation of heterotrophic microalgae.https://www.frontiersin.org/articles/10.3389/fmicb.2022.824189/fulladaptive laboratory evolutionCrypthecodinium cohniigrowthDHA contentfermentation supernatantquantitative proteomics |
| spellingShingle | Liangsen Liu Liangsen Liu Liangsen Liu Jinjin Diao Jinjin Diao Jinjin Diao Yali Bi Yali Bi Yali Bi Lei Zeng Lei Zeng Lei Zeng Fangzhong Wang Fangzhong Wang Fangzhong Wang Fangzhong Wang Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory Evolution Frontiers in Microbiology adaptive laboratory evolution Crypthecodinium cohnii growth DHA content fermentation supernatant quantitative proteomics |
| title | Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory Evolution |
| title_full | Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory Evolution |
| title_fullStr | Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory Evolution |
| title_full_unstemmed | Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory Evolution |
| title_short | Rewiring the Metabolic Network to Increase Docosahexaenoic Acid Productivity in Crypthecodinium cohnii by Fermentation Supernatant-Based Adaptive Laboratory Evolution |
| title_sort | rewiring the metabolic network to increase docosahexaenoic acid productivity in crypthecodinium cohnii by fermentation supernatant based adaptive laboratory evolution |
| topic | adaptive laboratory evolution Crypthecodinium cohnii growth DHA content fermentation supernatant quantitative proteomics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.824189/full |
| work_keys_str_mv | AT liangsenliu rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT liangsenliu rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT liangsenliu rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT jinjindiao rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT jinjindiao rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT jinjindiao rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT yalibi rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT yalibi rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT yalibi rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT leizeng rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT leizeng rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT leizeng rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT fangzhongwang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT fangzhongwang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT fangzhongwang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT fangzhongwang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT leichen rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT leichen rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT leichen rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT weiwenzhang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT weiwenzhang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT weiwenzhang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT weiwenzhang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution AT weiwenzhang rewiringthemetabolicnetworktoincreasedocosahexaenoicacidproductivityincrypthecodiniumcohniibyfermentationsupernatantbasedadaptivelaboratoryevolution |