Protocol for identifying stressed granulocytes from septic mice

Summary: Sepsis trains stressed granulocytes to boost nonspecific response and trigger a new wave of inflammation when facing secondary infection. Here, we present a protocol for a murine model of sepsis with secondary infection. We describe steps for cecal ligation and puncture operation and rechal...

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Bibliographic Details
Main Authors: Yu Hao, Can Zhang, Fangyuan Li, Yang Fan, Kexin Chi, Hui Zeng, Ju Zhang
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001230