Breaking the Restriction Barriers and Applying CRISPRi as a Gene Silencing Tool in <i>Pseudoclostridium thermosuccinogenes</i>

Breaking the Restriction Barriers and Applying CRISPRi as a Gene Silencing Tool in <i>Pseudoclostridium thermosuccinogenes</i>

<i>Pseudoclostridium thermosuccinogenes</i> is a thermophilic bacterium capable of producing succinate from lignocellulosic-derived sugars and has the potential to be exploited as a platform organism. However, exploitation of <i>P. thermosuccinogenes</i> has been limited part...

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Bibliographic Details
Main Authors: Joyshree Ganguly, Maria Martin-Pascual, Diego Montiel González, Alkan Bulut, Bram Vermeulen, Ivo Tjalma, Athina Vidaki, Richard van Kranenburg
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Microorganisms
Subjects:
<i>Pseudoclostridium thermosuccinogenes</i>
genetic accessibility
in vivo methylation
CRISPRi
malic enzyme
Online Access:https://www.mdpi.com/2076-2607/10/4/698

Internet

https://www.mdpi.com/2076-2607/10/4/698

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