Sperm induction of somatic cell-cell fusion as a novel functional test
The fusion of mammalian gametes requires the interaction between IZUMO1 on the sperm and JUNO on the oocyte. We have recently shown that ectopic expression of mouse IZUMO1 induces cell-cell fusion and that sperm can fuse to fibroblasts expressing JUNO. Here, we found that the incubation of mouse spe...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2024-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/94228 |
| _version_ | 1797300095476039680 |
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| author | Nicolas G Brukman Clari Valansi Benjamin Podbilewicz |
| author_facet | Nicolas G Brukman Clari Valansi Benjamin Podbilewicz |
| author_sort | Nicolas G Brukman |
| collection | DOAJ |
| description | The fusion of mammalian gametes requires the interaction between IZUMO1 on the sperm and JUNO on the oocyte. We have recently shown that ectopic expression of mouse IZUMO1 induces cell-cell fusion and that sperm can fuse to fibroblasts expressing JUNO. Here, we found that the incubation of mouse sperm with hamster fibroblasts or human epithelial cells in culture induces the fusion between these somatic cells and the formation of syncytia, a pattern previously observed with some animal viruses. This sperm-induced cell-cell fusion requires a species-matching JUNO on both fusing cells, can be blocked by an antibody against IZUMO1, and does not rely on the synthesis of new proteins. The fusion is dependent on the sperm’s fusogenic capacity, making this a reliable, fast, and simple method for predicting sperm function during the diagnosis of male infertility. |
| first_indexed | 2024-03-07T23:01:18Z |
| format | Article |
| id | doaj.art-96224c3a73184e5497af9447dde3beca |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-07T23:01:18Z |
| publishDate | 2024-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-96224c3a73184e5497af9447dde3beca2024-02-22T13:41:55ZengeLife Sciences Publications LtdeLife2050-084X2024-01-011310.7554/eLife.94228Sperm induction of somatic cell-cell fusion as a novel functional testNicolas G Brukman0https://orcid.org/0000-0002-9865-2503Clari Valansi1Benjamin Podbilewicz2https://orcid.org/0000-0002-0411-4182Department of Biology, Technion-Israel Institute of Technology, Haifa, IsraelDepartment of Biology, Technion-Israel Institute of Technology, Haifa, IsraelDepartment of Biology, Technion-Israel Institute of Technology, Haifa, IsraelThe fusion of mammalian gametes requires the interaction between IZUMO1 on the sperm and JUNO on the oocyte. We have recently shown that ectopic expression of mouse IZUMO1 induces cell-cell fusion and that sperm can fuse to fibroblasts expressing JUNO. Here, we found that the incubation of mouse sperm with hamster fibroblasts or human epithelial cells in culture induces the fusion between these somatic cells and the formation of syncytia, a pattern previously observed with some animal viruses. This sperm-induced cell-cell fusion requires a species-matching JUNO on both fusing cells, can be blocked by an antibody against IZUMO1, and does not rely on the synthesis of new proteins. The fusion is dependent on the sperm’s fusogenic capacity, making this a reliable, fast, and simple method for predicting sperm function during the diagnosis of male infertility.https://elifesciences.org/articles/94228male fertility testIzumo1Junoin vitro fertilizationegg-sperm fusionfusion from without |
| spellingShingle | Nicolas G Brukman Clari Valansi Benjamin Podbilewicz Sperm induction of somatic cell-cell fusion as a novel functional test eLife male fertility test Izumo1 Juno in vitro fertilization egg-sperm fusion fusion from without |
| title | Sperm induction of somatic cell-cell fusion as a novel functional test |
| title_full | Sperm induction of somatic cell-cell fusion as a novel functional test |
| title_fullStr | Sperm induction of somatic cell-cell fusion as a novel functional test |
| title_full_unstemmed | Sperm induction of somatic cell-cell fusion as a novel functional test |
| title_short | Sperm induction of somatic cell-cell fusion as a novel functional test |
| title_sort | sperm induction of somatic cell cell fusion as a novel functional test |
| topic | male fertility test Izumo1 Juno in vitro fertilization egg-sperm fusion fusion from without |
| url | https://elifesciences.org/articles/94228 |
| work_keys_str_mv | AT nicolasgbrukman sperminductionofsomaticcellcellfusionasanovelfunctionaltest AT clarivalansi sperminductionofsomaticcellcellfusionasanovelfunctionaltest AT benjaminpodbilewicz sperminductionofsomaticcellcellfusionasanovelfunctionaltest |