Clinical application of immune repertoire sequencing in solid organ transplant
BackgroundMeasurement of T cell receptor (TCR) or B cell receptor (BCR) gene utilization may be valuable in monitoring the dynamic changes in donor-reactive clonal populations following transplantation and enabling adjustment in therapy to avoid the consequences of excess immune suppression or to pr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1100479/full |
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| author | Paaksum Wong Davide P. Cina Karen R. Sherwood Karen R. Sherwood Franz Fenninger Franz Fenninger Ruth Sapir-Pichhadze Ruth Sapir-Pichhadze Constantin Polychronakos James Lan James Lan Paul A. Keown Paul A. Keown |
| author_facet | Paaksum Wong Davide P. Cina Karen R. Sherwood Karen R. Sherwood Franz Fenninger Franz Fenninger Ruth Sapir-Pichhadze Ruth Sapir-Pichhadze Constantin Polychronakos James Lan James Lan Paul A. Keown Paul A. Keown |
| author_sort | Paaksum Wong |
| collection | DOAJ |
| description | BackgroundMeasurement of T cell receptor (TCR) or B cell receptor (BCR) gene utilization may be valuable in monitoring the dynamic changes in donor-reactive clonal populations following transplantation and enabling adjustment in therapy to avoid the consequences of excess immune suppression or to prevent rejection with contingent graft damage and to indicate the development of tolerance.ObjectiveWe performed a review of current literature to examine research in immune repertoire sequencing in organ transplantation and to assess the feasibility of this technology for clinical application in immune monitoring.MethodsWe searched MEDLINE and PubMed Central for English-language studies published between 2010 and 2021 that examined T cell/B cell repertoire dynamics upon immune activation. Manual filtering of the search results was performed based on relevancy and predefined inclusion criteria. Data were extracted based on study and methodology characteristics.ResultsOur initial search yielded 1933 articles of which 37 met the inclusion criteria; 16 of these were kidney transplant studies (43%) and 21 were other or general transplantation studies (57%). The predominant method for repertoire characterization was sequencing the CDR3 region of the TCR β chain. Repertoires of transplant recipients were found to have decreased diversity in both rejectors and non-rejectors when compared to healthy controls. Rejectors and those with opportunistic infections were more likely to have clonal expansion in T or B cell populations. Mixed lymphocyte culture followed by TCR sequencing was used in 6 studies to define an alloreactive repertoire and in specialized transplant settings to track tolerance.ConclusionMethodological approaches to immune repertoire sequencing are becoming established and offer considerable potential as a novel clinical tool for pre- and post-transplant immune monitoring. |
| first_indexed | 2024-04-10T15:17:25Z |
| format | Article |
| id | doaj.art-9641b2ef54fb4d38998a894ab9993624 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T15:17:25Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-9641b2ef54fb4d38998a894ab99936242023-02-14T18:29:13ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-02-011410.3389/fimmu.2023.11004791100479Clinical application of immune repertoire sequencing in solid organ transplantPaaksum Wong0Davide P. Cina1Karen R. Sherwood2Karen R. Sherwood3Franz Fenninger4Franz Fenninger5Ruth Sapir-Pichhadze6Ruth Sapir-Pichhadze7Constantin Polychronakos8James Lan9James Lan10Paul A. Keown11Paul A. Keown12Department of Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Urologic Sciences, University of British Columbia, Vancouver, BC, CanadaDepartment of Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Medicine, Division of Nephrology, McGill University, Montreal, QC, CanadaDepartment of Epidemiology, Biostatistics and Occupational Health, McGill University, Montreal, QC, CanadaDepartment of Pediatrics, The Research Institute of the McGill University Health Centre and the Montreal Children’s Hospital, Montreal, QC, CanadaDepartment of Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Medicine, University of British Columbia, Vancouver, BC, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, CanadaBackgroundMeasurement of T cell receptor (TCR) or B cell receptor (BCR) gene utilization may be valuable in monitoring the dynamic changes in donor-reactive clonal populations following transplantation and enabling adjustment in therapy to avoid the consequences of excess immune suppression or to prevent rejection with contingent graft damage and to indicate the development of tolerance.ObjectiveWe performed a review of current literature to examine research in immune repertoire sequencing in organ transplantation and to assess the feasibility of this technology for clinical application in immune monitoring.MethodsWe searched MEDLINE and PubMed Central for English-language studies published between 2010 and 2021 that examined T cell/B cell repertoire dynamics upon immune activation. Manual filtering of the search results was performed based on relevancy and predefined inclusion criteria. Data were extracted based on study and methodology characteristics.ResultsOur initial search yielded 1933 articles of which 37 met the inclusion criteria; 16 of these were kidney transplant studies (43%) and 21 were other or general transplantation studies (57%). The predominant method for repertoire characterization was sequencing the CDR3 region of the TCR β chain. Repertoires of transplant recipients were found to have decreased diversity in both rejectors and non-rejectors when compared to healthy controls. Rejectors and those with opportunistic infections were more likely to have clonal expansion in T or B cell populations. Mixed lymphocyte culture followed by TCR sequencing was used in 6 studies to define an alloreactive repertoire and in specialized transplant settings to track tolerance.ConclusionMethodological approaches to immune repertoire sequencing are becoming established and offer considerable potential as a novel clinical tool for pre- and post-transplant immune monitoring.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1100479/fullsolid organ transplantalloimmunitylymphocyte receptor sequencingB cell receptor (BCR)T cell receptor (TCR) |
| spellingShingle | Paaksum Wong Davide P. Cina Karen R. Sherwood Karen R. Sherwood Franz Fenninger Franz Fenninger Ruth Sapir-Pichhadze Ruth Sapir-Pichhadze Constantin Polychronakos James Lan James Lan Paul A. Keown Paul A. Keown Clinical application of immune repertoire sequencing in solid organ transplant Frontiers in Immunology solid organ transplant alloimmunity lymphocyte receptor sequencing B cell receptor (BCR) T cell receptor (TCR) |
| title | Clinical application of immune repertoire sequencing in solid organ transplant |
| title_full | Clinical application of immune repertoire sequencing in solid organ transplant |
| title_fullStr | Clinical application of immune repertoire sequencing in solid organ transplant |
| title_full_unstemmed | Clinical application of immune repertoire sequencing in solid organ transplant |
| title_short | Clinical application of immune repertoire sequencing in solid organ transplant |
| title_sort | clinical application of immune repertoire sequencing in solid organ transplant |
| topic | solid organ transplant alloimmunity lymphocyte receptor sequencing B cell receptor (BCR) T cell receptor (TCR) |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1100479/full |
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