TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory Diseases
BackgroundThe prevalence of obesity has dramatically increased in children in the last few decades and is associated with chronic inflammatory diseases. Fat tissue produces IL-6 and TNF-α, which are stimuli for TH17 cell differentiation. These cells are characterized by expression of the transcripti...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01543/full |
| _version_ | 1818930624356941824 |
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| author | Theresa Isabell Schindler Johanna-Josophina Wagner Johanna-Josophina Wagner Sybelle Goedicke-Fritz Sybelle Goedicke-Fritz Tobias Rogosch Verena Coccejus Verena Laudenbach Wilfried Nikolaizik Christoph Härtel Rolf Felix Maier Sebastian Kerzel Michael Zemlin Michael Zemlin |
| author_facet | Theresa Isabell Schindler Johanna-Josophina Wagner Johanna-Josophina Wagner Sybelle Goedicke-Fritz Sybelle Goedicke-Fritz Tobias Rogosch Verena Coccejus Verena Laudenbach Wilfried Nikolaizik Christoph Härtel Rolf Felix Maier Sebastian Kerzel Michael Zemlin Michael Zemlin |
| author_sort | Theresa Isabell Schindler |
| collection | DOAJ |
| description | BackgroundThe prevalence of obesity has dramatically increased in children in the last few decades and is associated with chronic inflammatory diseases. Fat tissue produces IL-6 and TNF-α, which are stimuli for TH17 cell differentiation. These cells are characterized by expression of the transcription factor receptor-related orphan receptor C (RORC) and by IL-17A production. In murine models, obesity has been linked with elevated TH17 cell frequencies. The aim of this study was to explore whether being overweight was associated with an elevated frequency of circulating TH17 cells or elevated messenger RNA (mRNA)-levels of IL-17A and RORC in children without chronic inflammatory diseases.MethodsWe studied peripheral blood samples from 15 overweight and 50 non-overweight children without a history of autoimmune diseases, asthma, atopic dermatitis or allergic rhinoconjunctivitis. TH17 cells were quantified in Ionomycin stimulated peripheral blood mononuclear cells by flow cytometry using intracellular IL-17A staining. RORC- and IL-17A expressions were measured by real-time PCR.ResultsWe found significantly elevated TH cell frequencies in overweight children compared then on-overweight controls with 34.7 ± 1.5% of CD3+CD4+ cells versus 25.4 ± 2.4% (mean ± SEM, p = 0.0023), respectively. Moreover, TH cell frequencies correlated positively with body mass index (r = 0.42, p = 0.0005, respectively). The relative mRNA expression of RORC (p = 0.013) and IL-17A (p = 0.014) were upregulated in overweight compared to non-overweight children.ConclusionChildhood obesity is an independent factor that is associated with an elevated frequency of circulating TH17 cells and higher expression of RORC- and IL-17A-mRNA after in vitro stimulation with Ionomycin. This might be due to the inflammatory activity of the fat tissue. Studies on TH17 immunity should not only be adjusted for acute and chronic inflammatory diseases but also for overweight. |
| first_indexed | 2024-12-20T04:03:39Z |
| format | Article |
| id | doaj.art-96460293009e455081e6b0710f35fa43 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T04:03:39Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-96460293009e455081e6b0710f35fa432022-12-21T19:54:06ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-11-01810.3389/fimmu.2017.01543298555TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory DiseasesTheresa Isabell Schindler0Johanna-Josophina Wagner1Johanna-Josophina Wagner2Sybelle Goedicke-Fritz3Sybelle Goedicke-Fritz4Tobias Rogosch5Verena Coccejus6Verena Laudenbach7Wilfried Nikolaizik8Christoph Härtel9Rolf Felix Maier10Sebastian Kerzel11Michael Zemlin12Michael Zemlin13Children’s Hospital, Philipps University, Marburg, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyDepartment of Pediatrics, Helios-Klinikum Buch, Berlin, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyDepartment of General Pediatrics and Neonatology, University Children’s Hospital of Saarland, Homburg, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyDepartment of Pediatrics, University of Lübeck, Lübeck, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyDepartment of Pediatric Pneumology and Allergy, University Children’s Hospital Regensburg, Regensburg, GermanyChildren’s Hospital, Philipps University, Marburg, GermanyDepartment of General Pediatrics and Neonatology, University Children’s Hospital of Saarland, Homburg, GermanyBackgroundThe prevalence of obesity has dramatically increased in children in the last few decades and is associated with chronic inflammatory diseases. Fat tissue produces IL-6 and TNF-α, which are stimuli for TH17 cell differentiation. These cells are characterized by expression of the transcription factor receptor-related orphan receptor C (RORC) and by IL-17A production. In murine models, obesity has been linked with elevated TH17 cell frequencies. The aim of this study was to explore whether being overweight was associated with an elevated frequency of circulating TH17 cells or elevated messenger RNA (mRNA)-levels of IL-17A and RORC in children without chronic inflammatory diseases.MethodsWe studied peripheral blood samples from 15 overweight and 50 non-overweight children without a history of autoimmune diseases, asthma, atopic dermatitis or allergic rhinoconjunctivitis. TH17 cells were quantified in Ionomycin stimulated peripheral blood mononuclear cells by flow cytometry using intracellular IL-17A staining. RORC- and IL-17A expressions were measured by real-time PCR.ResultsWe found significantly elevated TH cell frequencies in overweight children compared then on-overweight controls with 34.7 ± 1.5% of CD3+CD4+ cells versus 25.4 ± 2.4% (mean ± SEM, p = 0.0023), respectively. Moreover, TH cell frequencies correlated positively with body mass index (r = 0.42, p = 0.0005, respectively). The relative mRNA expression of RORC (p = 0.013) and IL-17A (p = 0.014) were upregulated in overweight compared to non-overweight children.ConclusionChildhood obesity is an independent factor that is associated with an elevated frequency of circulating TH17 cells and higher expression of RORC- and IL-17A-mRNA after in vitro stimulation with Ionomycin. This might be due to the inflammatory activity of the fat tissue. Studies on TH17 immunity should not only be adjusted for acute and chronic inflammatory diseases but also for overweight.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01543/fullTH17 cellsIL-17Areceptor-related orphan receptor Cinflammationoverweightchildren |
| spellingShingle | Theresa Isabell Schindler Johanna-Josophina Wagner Johanna-Josophina Wagner Sybelle Goedicke-Fritz Sybelle Goedicke-Fritz Tobias Rogosch Verena Coccejus Verena Laudenbach Wilfried Nikolaizik Christoph Härtel Rolf Felix Maier Sebastian Kerzel Michael Zemlin Michael Zemlin TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory Diseases Frontiers in Immunology TH17 cells IL-17A receptor-related orphan receptor C inflammation overweight children |
| title | TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory Diseases |
| title_full | TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory Diseases |
| title_fullStr | TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory Diseases |
| title_full_unstemmed | TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory Diseases |
| title_short | TH17 Cell Frequency in Peripheral Blood Is Elevated in Overweight Children without Chronic Inflammatory Diseases |
| title_sort | th17 cell frequency in peripheral blood is elevated in overweight children without chronic inflammatory diseases |
| topic | TH17 cells IL-17A receptor-related orphan receptor C inflammation overweight children |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01543/full |
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