Cy5 labeled single-stranded DNA-polydopamine nanoparticle conjugate-based FRET assay for reactive oxygen species detection

Cy5 labeled single-stranded DNA-polydopamine nanoparticle conjugate-based FRET assay for reactive oxygen species detection

This work reports on a simple and feasible fluorescence resonance energy transfer (FRET) assay for detecting reactive oxygen species (ROS) both in solution and living cell using polydopamine nanoparticle (PDA NP) as energy acceptor and Cy5 labeled single-stranded DNA (Cy5-ssDNA) as energy donor. The...

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Bibliographic Details
Main Authors: Lina Ma, Zhen Lei, Fuyao Liu, Zhenxin Wang
Format: Article
Language:English
Published: Elsevier 2015-03-01
Series:Sensing and Bio-Sensing Research
Subjects:
Polydopamine nanoparticles
Cy5 labeled single-stranded DNA
Reactive oxygen species
Fluorescence resonance energy transfer
Living cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2214180415000021

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http://www.sciencedirect.com/science/article/pii/S2214180415000021

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