Quantitative determination of fluorescence labeling implemented in cell cultures

Abstract Background Labeling efficiency is a crucial parameter in fluorescence applications, especially when studying biomolecular interactions. Current approaches for estimating the yield of fluorescent labeling have critical drawbacks that usually lead them to be inaccurate or not quantitative. Re...

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Bibliographic Details
Main Authors: Chiara Schirripa Spagnolo, Aldo Moscardini, Rosy Amodeo, Fabio Beltram, Stefano Luin
Format: Article
Language:English
Published: BMC 2023-09-01
Series:BMC Biology
Subjects:
Online Access:https://doi.org/10.1186/s12915-023-01685-0