Quantitative determination of fluorescence labeling implemented in cell cultures
Abstract Background Labeling efficiency is a crucial parameter in fluorescence applications, especially when studying biomolecular interactions. Current approaches for estimating the yield of fluorescent labeling have critical drawbacks that usually lead them to be inaccurate or not quantitative. Re...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-09-01
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Series: | BMC Biology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12915-023-01685-0 |