Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis
Abstract Background Postmenopausal osteoporosis (PMO) that results from estrogen withdrawal is the most common primary osteoporosis among older women. However, little is known about the mechanism of PMO, and effective treatment of PMO is limited. Methods We used real-time polymerase chain reaction (...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-020-02160-0 |
| _version_ | 1798035263632441344 |
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| author | Lu Li Xin Zhou Jun-tao Zhang Ai-feng Liu Chao Zhang Jin-chang Han Xiao-qing Zhang Si Wu Xiao-yu Zhang Fu-quan Lv |
| author_facet | Lu Li Xin Zhou Jun-tao Zhang Ai-feng Liu Chao Zhang Jin-chang Han Xiao-qing Zhang Si Wu Xiao-yu Zhang Fu-quan Lv |
| author_sort | Lu Li |
| collection | DOAJ |
| description | Abstract Background Postmenopausal osteoporosis (PMO) that results from estrogen withdrawal is the most common primary osteoporosis among older women. However, little is known about the mechanism of PMO, and effective treatment of PMO is limited. Methods We used real-time polymerase chain reaction (qPCR), Western blotting, and RNA pull down to investigate the relationship between miR-186 and MOB Kinase Activator 1A (Mob1). Also, we investigated the effect of exosome in osteogenesis using alkaline phosphatase (ALP) staining. And hematoxylin eosin (HE) staining was used to verify the osteogenesis in PMO model. Results Exosomal miR-186 plays an important role in bone formation. The results of miRNA-seq and q-PCR showed that miR-186 was upregulated in a PMO + Exo treatment group. Results of RNA-pull down and luciferase reporter assays verified interactions between miR-186 and Mob1. We also verified the Hippo signaling pathway plays an important role in osteogenesis. Conclusions We concluded that exosomes derived from human bone marrow mesenchymal stem cells (hBMSCs) can transfer miR-186 to promote osteogenesis in ovariectomy (OVX) rats through the Hippo signaling pathway. |
| first_indexed | 2024-04-11T20:55:47Z |
| format | Article |
| id | doaj.art-968028e0320e46c89a5429ca2ac338b9 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T20:55:47Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-968028e0320e46c89a5429ca2ac338b92022-12-22T04:03:42ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2021-01-0116111010.1186/s13018-020-02160-0Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosisLu Li0Xin Zhou1Jun-tao Zhang2Ai-feng Liu3Chao Zhang4Jin-chang Han5Xiao-qing Zhang6Si Wu7Xiao-yu Zhang8Fu-quan Lv9Department of Acupuncture, Tianjin Nankai HospitalDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese MedicineDepartment of Acupuncture, Tianjin Nankai HospitalAbstract Background Postmenopausal osteoporosis (PMO) that results from estrogen withdrawal is the most common primary osteoporosis among older women. However, little is known about the mechanism of PMO, and effective treatment of PMO is limited. Methods We used real-time polymerase chain reaction (qPCR), Western blotting, and RNA pull down to investigate the relationship between miR-186 and MOB Kinase Activator 1A (Mob1). Also, we investigated the effect of exosome in osteogenesis using alkaline phosphatase (ALP) staining. And hematoxylin eosin (HE) staining was used to verify the osteogenesis in PMO model. Results Exosomal miR-186 plays an important role in bone formation. The results of miRNA-seq and q-PCR showed that miR-186 was upregulated in a PMO + Exo treatment group. Results of RNA-pull down and luciferase reporter assays verified interactions between miR-186 and Mob1. We also verified the Hippo signaling pathway plays an important role in osteogenesis. Conclusions We concluded that exosomes derived from human bone marrow mesenchymal stem cells (hBMSCs) can transfer miR-186 to promote osteogenesis in ovariectomy (OVX) rats through the Hippo signaling pathway.https://doi.org/10.1186/s13018-020-02160-0Postmenopausal osteoporosisExosomesmiRNAOsteogenesis |
| spellingShingle | Lu Li Xin Zhou Jun-tao Zhang Ai-feng Liu Chao Zhang Jin-chang Han Xiao-qing Zhang Si Wu Xiao-yu Zhang Fu-quan Lv Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis Journal of Orthopaedic Surgery and Research Postmenopausal osteoporosis Exosomes miRNA Osteogenesis |
| title | Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis |
| title_full | Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis |
| title_fullStr | Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis |
| title_full_unstemmed | Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis |
| title_short | Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis |
| title_sort | exosomal mir 186 derived from bmscs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis |
| topic | Postmenopausal osteoporosis Exosomes miRNA Osteogenesis |
| url | https://doi.org/10.1186/s13018-020-02160-0 |
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