Detection of Escherichia Coli Using PCR Analysis Without DNA Extraction

This study aimed to detect Escherichia coli directly without DNA extraction. The nucleus membrane and cell membranes of the Escherichia coli are composed of a phospholipid bilayer, damaged if heated at 950C. Pre-denaturation and denaturation of PCR were carried out at 950C. The two stages are though...

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Bibliographic Details
Main Authors: Wimbuh Tri Widodo, Choirul Huda
Format: Article
Language:English
Published: Universitas Airlangga 2021-06-01
Series:Folia Medica Indonesiana
Subjects:
Online Access:https://e-journal.unair.ac.id/FMI/article/view/22097