Mapping germ-layer specification preventing genes in hPSCs via genome-scale CRISPR screening

Mapping germ-layer specification preventing genes in hPSCs via genome-scale CRISPR screening

Summary: Understanding the biological processes that determine the entry of three germ layers of human pluripotent stem cells (hPSCs) is a central question in developmental and stem cell biology. Here, we genetically engineered hPSCs with the germ layer reporter and inducible CRISPR/Cas9 knockout sy...

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Bibliographic Details
Main Authors: Xiangjie Xu, Yanhua Du, Lin Ma, Shuwei Zhang, Lei Shi, Zhenyu Chen, Zhongshu Zhou, Yi Hui, Yang Liu, Yujiang Fang, Beibei Fan, Zhongliang Liu, Nan Li, Shanshan Zhou, Cizhong Jiang, Ling Liu, Xiaoqing Zhang
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:iScience
Subjects:
Biological Sciences
Cell Biology
Stem Cells Research
Developmental Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004220311238

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http://www.sciencedirect.com/science/article/pii/S2589004220311238

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