A new isolation method for bacterial extracellular vesicles providing greater purity and improved proteomic detection of vesicle proteins

Abstract Contaminants within cell culture media often co‐isolate with eukaryotic extracellular vesicles (EVs) thus affecting their biological properties. It has yet to be investigated if this is also true for bacterial EVs (BEVs), especially for organisms grown in complex culture media containing an...

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Bibliographic Details
Main Authors: Lena Hoang My Le, Joel R. Steele, Le Ying, Ralf B. Schittenhelm, Richard L. Ferrero
Format: Article
Language:English
Published: Wiley 2023-05-01
Series:Journal of Extracellular Biology
Subjects:
Online Access:https://doi.org/10.1002/jex2.84