Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat
Salmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most pr...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação APINCO de Ciência e Tecnologia Avícolas
2014-03-01
|
| Series: | Brazilian Journal of Poultry Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100006&lng=en&tlng=en |
| _version_ | 1818852363411128320 |
|---|---|
| author | FL Colla L Mion L Parizotto LB Rodrigues F Pilotto EL Dickel VP do Nascimento LR dos Santos |
| author_facet | FL Colla L Mion L Parizotto LB Rodrigues F Pilotto EL Dickel VP do Nascimento LR dos Santos |
| author_sort | FL Colla |
| collection | DOAJ |
| description | Salmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most probable number (mMPN) methods, which use microplates, can be adapted for the enumeration of bacteria, saving up time and materials. The aim of the present paper is to assess two mMPN methods for the enumeration of Salmonella sp in artificially-contaminated chicken meat samples. Microplates containing 24 wells (method A) and 96 wells (method B), both with peptone water as pre-enrichment medium and modified semi-solid Rappaport-Vassiliadis (MSRV) as selective enrichment medium, were used. The meat matrix consisted of 25g of autoclaved ground chicken breast contaminated with dilutions of up to 10(6) of Salmonella Typhimurium (ST) and Escherichia coli (EC). In method A, the dilution 10-5 of Salmonella Typhimurium corresponded to >57 MPN/mL and the dilution 10-6 was equal to 30 MPN/mL. There was a correlation between the counts used for the artificial contamination of the samples and those recovered by mMPN, indicating that the method A was sensitive for the enumeration of different levels of contamination of the meat matrix. In method B, there was no correlation between the inoculated dilutions and the mMPN results. |
| first_indexed | 2024-12-19T07:19:44Z |
| format | Article |
| id | doaj.art-96f3e998188e44ffab665bfc0b09aa03 |
| institution | Directory Open Access Journal |
| issn | 1806-9061 |
| language | English |
| last_indexed | 2024-12-19T07:19:44Z |
| publishDate | 2014-03-01 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj.art-96f3e998188e44ffab665bfc0b09aa032022-12-21T20:30:58ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612014-03-01161454810.1590/S1516-635X2014000100006S1516-635X2014000100006Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meatFL Colla0L Mion1L Parizotto2LB Rodrigues3F Pilotto4EL Dickel5VP do Nascimento6LR dos Santos7Universidade de Passo FundoUniversidade de Passo FundoUniversidade de Passo FundoUniversidade de Passo FundoUniversidade de Passo FundoUniversidade de Passo FundoUniversidade Federal do Rio Grande do SulUniversidade de Passo FundoSalmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most probable number (mMPN) methods, which use microplates, can be adapted for the enumeration of bacteria, saving up time and materials. The aim of the present paper is to assess two mMPN methods for the enumeration of Salmonella sp in artificially-contaminated chicken meat samples. Microplates containing 24 wells (method A) and 96 wells (method B), both with peptone water as pre-enrichment medium and modified semi-solid Rappaport-Vassiliadis (MSRV) as selective enrichment medium, were used. The meat matrix consisted of 25g of autoclaved ground chicken breast contaminated with dilutions of up to 10(6) of Salmonella Typhimurium (ST) and Escherichia coli (EC). In method A, the dilution 10-5 of Salmonella Typhimurium corresponded to >57 MPN/mL and the dilution 10-6 was equal to 30 MPN/mL. There was a correlation between the counts used for the artificial contamination of the samples and those recovered by mMPN, indicating that the method A was sensitive for the enumeration of different levels of contamination of the meat matrix. In method B, there was no correlation between the inoculated dilutions and the mMPN results.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100006&lng=en&tlng=enChicken meatmost probable numberSalmonella |
| spellingShingle | FL Colla L Mion L Parizotto LB Rodrigues F Pilotto EL Dickel VP do Nascimento LR dos Santos Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat Brazilian Journal of Poultry Science Chicken meat most probable number Salmonella |
| title | Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat |
| title_full | Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat |
| title_fullStr | Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat |
| title_full_unstemmed | Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat |
| title_short | Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat |
| title_sort | miniaturized most probable number for the enumeration of salmonella sp in artificially contaminated chicken meat |
| topic | Chicken meat most probable number Salmonella |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100006&lng=en&tlng=en |
| work_keys_str_mv | AT flcolla miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat AT lmion miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat AT lparizotto miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat AT lbrodrigues miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat AT fpilotto miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat AT eldickel miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat AT vpdonascimento miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat AT lrdossantos miniaturizedmostprobablenumberfortheenumerationofsalmonellaspinartificiallycontaminatedchickenmeat |