Whole-mount in situ hybridization of small invertebrate embryos using laboratory mini-columns
To facilitate the handling of small invertebrate embryos during whole-mount in situ hybridization, a method of solution exchange using laboratory mini-columns was developed. This protocol speeds time-consuming aspiration of buffers, and avoids accidental loss of the embryos, by gently pushing soluti...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2007-12-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112617 |