Pharmacokinetics of a liposomal formulation of doxorubicin in rats
Background: Measuring free drug concentration following systemic administration of a liposomal drug is a crucial aspect of the assessment of its in vivo behavior. Therefore we require an efficient method to separate free drug in the plasma from encapsulated drug. Objectives: To study the pharmacokin...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-05-01
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| Series: | Saudi Pharmaceutical Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S131901641730083X |
| _version_ | 1818994727622541312 |
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| author | Zongyu Liu Ye Bi Yating Sun Fei Hao Jiahui Lu Qingfan Meng Robert J. Lee Yaping Tian Jing Xie |
| author_facet | Zongyu Liu Ye Bi Yating Sun Fei Hao Jiahui Lu Qingfan Meng Robert J. Lee Yaping Tian Jing Xie |
| author_sort | Zongyu Liu |
| collection | DOAJ |
| description | Background: Measuring free drug concentration following systemic administration of a liposomal drug is a crucial aspect of the assessment of its in vivo behavior. Therefore we require an efficient method to separate free drug in the plasma from encapsulated drug. Objectives: To study the pharmacokinetics of free doxorubicin (DOX) released from liposomal doxorubicin (L-DOX) in rats. Methods: L-DOX was prepared with encapsulation efficiency of 90% and was injected intravenously into rats. A solid-phase extraction (SPE) method coupled with UPLC–MS/MS was used to measure the concentration of F-DOX in rat plasma without disrupting the integrity of L-DOX. Results: This method exhibited a linear range of F-DOX from 0.2 to 200 ng/mL. Recovery, precision, linearity and accuracy of this technique appear satisfactory for pharmacokinetic study. The constituents of F-DOX ranged from 5.35% to 14.09% of total DOX in plasma at each time point measured after L-DOX administration. Conclusion: SPE method was suitable for studying the pharmacokinetics of F-DOX in rats receiving L-DOX. Keywords: Pharmacokinetics, Doxorubicin, Liposomes, Solid-phase extraction |
| first_indexed | 2024-12-20T21:02:33Z |
| format | Article |
| id | doaj.art-9720d28f73b54444b3e6a2f3f6928dd5 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-12-20T21:02:33Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-9720d28f73b54444b3e6a2f3f6928dd52022-12-21T19:26:41ZengElsevierSaudi Pharmaceutical Journal1319-01642017-05-01254531536Pharmacokinetics of a liposomal formulation of doxorubicin in ratsZongyu Liu0Ye Bi1Yating Sun2Fei Hao3Jiahui Lu4Qingfan Meng5Robert J. Lee6Yaping Tian7Jing Xie8Department of Clinical Medicine, Jilin University Bethune School of Medicine, Changchun 130021, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, China; Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai 519041, China; Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USADepartment of Dermatology and Venerology, 1st Hospital of Jilin University, Changchun 130061, China; Corresponding authors at: School of Life Sciences, Jilin University, Changchun 130012, China (J. Xie).School of Life Sciences, Jilin University, Changchun 130012, China; Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai 519041, China; Corresponding authors at: School of Life Sciences, Jilin University, Changchun 130012, China (J. Xie).Background: Measuring free drug concentration following systemic administration of a liposomal drug is a crucial aspect of the assessment of its in vivo behavior. Therefore we require an efficient method to separate free drug in the plasma from encapsulated drug. Objectives: To study the pharmacokinetics of free doxorubicin (DOX) released from liposomal doxorubicin (L-DOX) in rats. Methods: L-DOX was prepared with encapsulation efficiency of 90% and was injected intravenously into rats. A solid-phase extraction (SPE) method coupled with UPLC–MS/MS was used to measure the concentration of F-DOX in rat plasma without disrupting the integrity of L-DOX. Results: This method exhibited a linear range of F-DOX from 0.2 to 200 ng/mL. Recovery, precision, linearity and accuracy of this technique appear satisfactory for pharmacokinetic study. The constituents of F-DOX ranged from 5.35% to 14.09% of total DOX in plasma at each time point measured after L-DOX administration. Conclusion: SPE method was suitable for studying the pharmacokinetics of F-DOX in rats receiving L-DOX. Keywords: Pharmacokinetics, Doxorubicin, Liposomes, Solid-phase extractionhttp://www.sciencedirect.com/science/article/pii/S131901641730083X |
| spellingShingle | Zongyu Liu Ye Bi Yating Sun Fei Hao Jiahui Lu Qingfan Meng Robert J. Lee Yaping Tian Jing Xie Pharmacokinetics of a liposomal formulation of doxorubicin in rats Saudi Pharmaceutical Journal |
| title | Pharmacokinetics of a liposomal formulation of doxorubicin in rats |
| title_full | Pharmacokinetics of a liposomal formulation of doxorubicin in rats |
| title_fullStr | Pharmacokinetics of a liposomal formulation of doxorubicin in rats |
| title_full_unstemmed | Pharmacokinetics of a liposomal formulation of doxorubicin in rats |
| title_short | Pharmacokinetics of a liposomal formulation of doxorubicin in rats |
| title_sort | pharmacokinetics of a liposomal formulation of doxorubicin in rats |
| url | http://www.sciencedirect.com/science/article/pii/S131901641730083X |
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