MATRIX platform to analyze translation machinery remodeling in glioblastoma cells

Summary: Here, we present a protocol using MATRIX (mass spectrometry analysis of active translation factors using ribosome density fractionation and isotopic labeling experiments) platform to investigate changes of the protein synthesis machinery in U87MG glioblastoma cells in response to the rocagl...

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Bibliographic Details
Main Authors: J.J. David Ho, Tyler A. Cunningham, Jonathan R. Krieger, Jonathan H. Schatz, Stephen Lee
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007997