Gene doctoring: a method for recombineering in laboratory and pathogenic <it>Escherichia coli </it>strains
<p>Abstract</p> <p>Background</p> <p>Homologous recombination mediated by the λ-Red genes is a common method for making chromosomal modifications in <it>Escherichia coli</it>. Several protocols have been developed that differ in the mechanisms by which DNA,...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2009-12-01
|
Series: | BMC Microbiology |
Online Access: | http://www.biomedcentral.com/1471-2180/9/252 |