Gene doctoring: a method for recombineering in laboratory and pathogenic <it>Escherichia coli </it>strains

<p>Abstract</p> <p>Background</p> <p>Homologous recombination mediated by the λ-Red genes is a common method for making chromosomal modifications in <it>Escherichia coli</it>. Several protocols have been developed that differ in the mechanisms by which DNA,...

Full description

Bibliographic Details
Main Authors: Penn Charles W, Pallen Mark J, Heurlier Karin, Bingle Lewis EH, Lee David J, Busby Stephen JW, Hobman Jon L
Format: Article
Language:English
Published: BMC 2009-12-01
Series:BMC Microbiology
Online Access:http://www.biomedcentral.com/1471-2180/9/252