Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids

Abstract Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeede...

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Bibliographic Details
Main Authors: Ilya D. Solovyev, Alexandra V. Gavshina, Aditya S. Katti, Alexey I. Chizhik, Leonid M. Vinokurov, Grigory D. Lapshin, Tatiana V. Ivashina, Maria G. Khrenova, Igor I. Kireev, Ingo Gregor, Jörg Enderlein, Alexander P. Savitsky
Format: Article
Language:English
Published: Nature Portfolio 2018-10-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-018-33250-z