Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing

Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing

Abstract Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M...

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Bibliographic Details
Main Authors: Michael Alonge, Ludivine Lebeigle, Melanie Kirsche, Katie Jenike, Shujun Ou, Sergey Aganezov, Xingang Wang, Zachary B. Lippman, Michael C. Schatz, Sebastian Soyk
Format: Article
Language:English
Published: BMC 2022-12-01
Series:Genome Biology
Subjects:
Genome sequencing
Assembly scaffolding
Genome editing
Tomato
Online Access:https://doi.org/10.1186/s13059-022-02823-7

Internet

https://doi.org/10.1186/s13059-022-02823-7

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