Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal

Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal

To improve the characterization of snake venom protein profiles, we report the application of a new generation of proteomic methodology to deeply characterize complex protein mixtures. The new approach, combining a synergic multi-enzymatic and a time-limited digestion (MELD), is a versatile and stra...

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Bibliographic Details
Main Authors: Fernanda Gobbi Amorim, Damien Redureau, Thomas Crasset, Lou Freuville, Dominique Baiwir, Gabriel Mazzucchelli, Stefanie K. Menzies, Nicholas R. Casewell, Loïc Quinton
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:Toxins
Subjects:
venomics
proteomic
mass spectrometry
snake venom
toxin
multi-enzymatic digestion
Online Access:https://www.mdpi.com/2072-6651/15/6/357

Internet

https://www.mdpi.com/2072-6651/15/6/357

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